Synthesis of specific membrane proteins is a function of DNA replication an phospholipid synthesis in Caulobacter crescentus.
Synthesis of specific membrane proteins is a function of DNA replication an phospholipid synthesis in Caulobacter crescentus.
复制标题
新月柄杆菌中特定膜蛋白的合成是 DNA 复制和磷脂合成的功能。
DOI：
10.1016/0022-2836(82)90497-1
复制
发表时间：
1982
影响因子：
5.6
通讯作者：
Henry,S
中科院分区：
文献类型：
--
作者：
Shapiro,L;Mansour,J;Shaw,P;Henry,S
对新月曲杆菌改变磷脂合成对膜功能和蛋白质组成的影响的分析表明，与其他细菌一样，C。月牙草继续诱导乳糖转运系统并合成大多数膜蛋白。然而，我们表明，主要在跟踪细胞中合成的一组外膜蛋白的结合依赖于DNA复制，而DNA复制反过来又依赖于膜磷脂合成。此外，另一组膜蛋白的结合，其中两组主要在蜂拥细胞中合成，似乎与染色体的复制无关，但直接依赖于磷脂的合成。我们还发现，当磷脂合成被阻断时，鞭毛蛋白的合成受到抑制，这种影响可能是由DNA复制的初级抑制介导的。Newton提出的证据表明，鞭毛蛋白的合成依赖于DNA复制的特定执行点，这种联系可以作为差异蛋白合成的时间调节剂（Osleyet al., 1977; Sheffery & Newton, 1981）。我们认为，复制染色体和生长膜之间的直接联系可能反过来决定了新合成基因产物的膜组装位置。
Analysis of the effects on membrane function and protein composition of altering phospholipid synthesis inCaulobacter crescentusshowed that, like other bacteria,C. crescentuscontinues to induce a lactose transport system and to synthesize most membrane proteins. However, we show that the incorporation of a set of outer membrane proteins primarily synthesized in stalked cells is dependent on DNA replication which, in turn, is dependent on membrane phospholipid synthesis. Furthermore, the incorporation of another set of membrane proteins, two of which are synthesized primarily in the swarmer cell, appears to be independent of the replication of the chromosome but to be directly dependent on phospholipid synthesis. We have also found that when phospholipid synthesis is blocked, the synthesis of the flagellar proteins is inhibited and that this effect may be mediated by the primary inhibition of DNA replication. Newton has presented evidence that the synthesis of flagellar proteins is dependent on specific execution points in DNA replication and that this connection serves as a temporal regulator of differential protein synthesis (Osleyet al., 1977; Sheffery & Newton, 1981). We suggest here that a direct link between the replicating chromosome and the growing membrane might serve, in turn, to dictate the site of membrane assembly of newly synthesized gene products.