Hemorheological Approach to Improve Perfusion of Red Blood Cells with Reduced Deformability Using Drag-Reducing Polymer (In Vitro Study).
Hemorheological Approach to Improve Perfusion of Red Blood Cells with Reduced Deformability Using Drag-Reducing Polymer (In Vitro Study).
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DOI：
10.1097/mat.0000000000001559
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发表时间：
2022-05-01
期刊：
影响因子：
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通讯作者：
Kameneva MV
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文献类型：
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作者：
Crompton D;Gudla S;Waters JH;Sundd P;Kameneva MV
减阻聚合物（DRPs）是无毒的水溶性血液添加剂，当以纳摩尔浓度静脉内递送时，已显示其有益地改变血液动力学。本研究考察了DRPs通过分支微通道改变正常和不易变形的RBC混合物的交通的能力，并旨在支持和扩展直毛细管内的先前实验，以促进DRPs用于未来的临床使用。支化聚二甲基硅氧烷（PDMS）微通道灌注与正常牛红细胞的混合物，也含有热处理的变形较小的红细胞在30%的血细胞比容与10 ppm的DRP聚（环氧乙烷）（MW 4 M Da）。通过注射泵驱动悬浮液，在出口处收集，并在经受剪切应力时测量RBC尺寸以确定每个样品中健康RBC的比例。DRP消除了等离子体撇除现象的证据，并显着增加了微通道两端的压降。此外，发现DRP导致离开分支出口的健康RBC的比例从-8.5 ±2.5%（对照组）增加至+12.1 ± 5.4%（n=6，p=0.02）。这些结果表明DRP添加剂可用于改善体内不易变形的RBC的灌注，并表明其未来临床应用的潜力。
Drag-reducing polymers (DRPs) are nontoxic water-soluble blood additives which have been shown to beneficially alter hemodynamics when delivered intravenously in nanomolar concentrations. This study examines the ability of DRPs to alter the traffic of mixtures of normal and less-deformable RBCs through branched microchannels and is intended to support and expand upon previous experiments within straight capillary tubes to promote DRPs for future clinical use. Branched polydimethylsiloxane (PDMS) microchannels were perfused with a mixture of normal bovine RBCs also containing heat treated less-deformable RBCs at a hematocrit of 30% with 10ppm of the DRP poly(ethylene oxide) (MW 4M Da). Suspensions were driven by syringe pump, collected at outlets, and RBC dimensions measured while subject to shear stress to determine the proportion of healthy RBCs in each sample. DRPs eliminated evidence of the plasma skimming phenomena and significantly increased the pressure drop across microchannels. Further, DRPs were found to cause an increase in the proportion of healthy RBCs exiting the branch outlet from −8.5±2.5% (control groups) to +12.1±5.4% (n=6, p=0.02). These results suggest DRP additives may be used to improve the perfusion of less-deformable RBCs in vivo and indicates their potential for future clinical use.