SUC1 gene of Saccharomyces: a structural gene for the large (glycoprotein) and small (carbohydrate-free) forms of invertase.
SUC1 gene of Saccharomyces: a structural gene for the large (glycoprotein) and small (carbohydrate-free) forms of invertase.
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酵母菌的 SUC1 基因：大（糖蛋白）和小（无碳水化合物）转化酶的结构基因。
DOI：
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发表时间：
1981
影响因子：
5.3
通讯作者：
V. Mackay
中科院分区：
文献类型：
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作者：
L. Rodríguez;J. Lampen;V. Mackay
酿酒酵母回复突变株D10-ER 1已显示含有热敏形式的大（糖蛋白）和小（无碳水化合物）转化酶以及非常低水平的小酶，沿着野生型水平的大形式（T. Mizunaga等人，摩尔Cell. 1：460-468，1981）。这些特征共分离的回复菌株与野生型蔗糖发酵（SUC 1）或nonfermenting（suc 0）菌株的杂交。此外，在回复突变体D10-ER 1中，蔗糖和麦芽糖发酵之间存在紧密联系（SUC 1和MAL 1基因的特征）。由此我们推断，单一回复突变事件导致了D10-ER 1中观察到的几种变化，并且该突变定位在祖先菌株4059- 358 D中存在的SUC 1基因内或非常接近该基因。当D10-ER 1中的回复突变SUC 1等位基因（称为SUC 1-R1）与野生型SUC 1基因同时存在于二倍体细胞中时，其独立表达。仅携带野生型或突变基因的二倍体合成具有亲本Suc+单倍体特征的转化酶。通过适当的杂交排除了修饰基因对回复突变体D10-ER 1的转化酶的改变负责的可能性。我们的结论是，SUC 1是一个结构基因，编码的大型和小型形式的转化酶，并建议SUC 2通过SUC 5的结构基因以及。
Saccharomyces cerevisiae revertant strain D10-ER1 has been shown to contain thermosensitive forms of the large (glycoprotein) and small (carbohydrate-free) invertases and a very low level of the small enzyme, along with a wild-type level of the large form (T. Mizunaga et al., Mol. Cell. Biol. 1:460-468, 1981). These characteristics cosegregated in crosses of the revertant strain with wild-type sucrose-fermenting (SUC1) or nonfermenting (suc0) strains. In addition, there is tight linkage between sucrose and maltose fermentation in revertant D10-ER1 (characteristic of the SUC1 and MAL1 genes). From this we infer that a single reversion event is responsible for the several changes observed in D10-ER1, and that this mutation maps within or very close to the SUC1 gene present in the ancestor strain 4059-358D. The revertant SUC1 allele in D10-ER1 (termed SUC1-R1) was expressed independently of the wild-type SUC1 gene when both were present in diploid cells. Diploids carrying only the wild-type or the mutant genes synthesized invertases with the characteristics of the parental Suc+ haploids. The possibility that a modifier gene was responsible for the alterations in the invertases of revertant D10-ER1 was ruled out by appropriate crosses. We conclude that SUC1 is a structural gene that codes for both the large and the small forms of invertase and suggest that SUC2 through SUC5 are structural genes as well.