Generation and characterization of immortalized rat pancreatic stellate cells
Generation and characterization of immortalized rat pancreatic stellate cells
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DOI：
10.1152/ajpgi.00347.2003
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发表时间：
2004-07-01
影响因子：
4.5
通讯作者：
Emmrich, J
中科院分区：
文献类型：
--
作者：
Sparmann, G;Hohenadl, C;Emmrich, J
胰腺星状细胞（PSC）参与胰腺纤维化的发病机制等。在这里，我们提出的一代永生化的PSC分离后7和14天的逆转录病毒基因转移的SV 40大T抗原编码区。传代细胞系[大T永生化细胞（LTC）-7，LTC-14]在形态学、对调节细胞功能（如增殖）的介质的反应性和许多研究基因的表达谱方面保留了原代细胞的特征。而LTC-14保持了它们所组成的原代细胞的分化状态的形态特征，LTC-7看起来类似于早期阶段。因此，建立的细胞系代表了研究PSC生物学各个方面的通用工具。
Pancreatic stellate cells (PSCs) are involved in, among other things, the pathogenesis of pancreatic fibrosis. Here, we present the generation of immortalized PSCs 7 and 14 days after isolation by retroviral gene transfer of the SV40 large T antigen encoding region. Propagated cell lines [large T immortalized cells (LTC)-7, LTC-14] retained characteristics of primary cells in terms of morphology, responsiveness to mediators regulating cellular functions such as proliferation, and expression profile of a number of investigated genes. Whereas LTC-14 kept the morphological features of the differentiation status of the primary cells they were made of, LTC-7 appeared similar to an earlier stage. Thus the established cell lines represent a versatile tool to investigate various aspects of PSC biology.