EFFECTS OF THE SIZE AND NUMBER OF ZONA-PELLUCIDA OPENINGS ON HATCHING AND TROPHOBLAST OUTGROWTH IN THE MOUSE EMBRYO
EFFECTS OF THE SIZE AND NUMBER OF ZONA-PELLUCIDA OPENINGS ON HATCHING AND TROPHOBLAST OUTGROWTH IN THE MOUSE EMBRYO
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DOI：
10.1002/mrd.1080300110
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发表时间：
1991-09-01
影响因子：
2.5
通讯作者：
FELDBERG, D
中科院分区：
文献类型：
--
作者：
COHEN, J;FELDBERG, D
本研究旨在探讨卵透明膜（ZP）人工孔的大小和数量对体外孵化和滋养层生长的影响。 有限部分髓内剥离（PZD）产生小而窄的切口，并使用酸性台氏（AT）在ZP的较大区域进行钻孔以产生更大的圆孔。 一些胚胎被显微操作一次，另一些则被显微操作多次。 囊胚孵化优先通过人工间隙，但完成孵化是依赖于孔的大小。 只有16%（26/167）的PZD胚胎迁移通过狭窄的洞完全孵化，其余的被困在一个典型的数字8形状。 72%（43/60）的那些通过较大的PZD孔迁移孵化，但没有观察到滋养层生长。 显著（P < 0.001）更多的AT囊胚孵化（248/270; 92%），并显示滋养层生长（176/248; 70%）。 在AT胚胎中很少观察到通过几个开口同时孵化（14/167; 8%），但在36%（73/201）的PZD胚胎中确实发生了这种情况。 通过在ZP上的其他地方用AT钻孔，几乎可以完全避免PZD胚胎的捕获。 在其zonae多孔的胚胎优先孵化通过最大的开口。 结果表明，显微手术处理的人类胚胎在体外完全孵化的能力，应仔细（重新）评估临床显微操作系统的应用之前。 显微操作的胚胎，在其zonae小孔可以通过执行一个额外的更积极的开放程序在ZP的其他地方获救。
The present studies were performed to establish the effects of the size and number of artificial holes produced in the zona pellucida (ZP) on hatching and trophoblast outgrowth in vitro. Limited partial zona dissection (PZD) produced small, narrow incisions, and zona drilling with acidic Tyrode's (AT) across a larger area in the ZP was used to produce bigger round holes. Some embryos were micromanipulated once; others were micromanipulated several times. Blastocysts hatched preferentially through the artificial gaps, but completion of hatching was dependent on the size of the hole. Only 16% (26/167) of PZD embryos migrating through narrow holes hatched completely; the remainder were trapped in a typical figure-eight shape. Seventy-two percent (43/60) of those migrating through larger PZD holes hatched, but trophoblast outgrowth was not observed. Significantly (P < 0.001) more AT-blastocysts hatched (248/270; 92%) and showed trophoblast outgrowth (176/248; 70%). Simultaneous hatching through several openings was rarely observed in AT-embryos (14/167; 8%), but this did occur in 36% (73/201) of the PZD embryos. Trapping of PZD-embryos could be almost entirely avoided by drilling with AT elsewhere on the ZP. Embryos with multiple holes in their zonae preferentially hatched through the largest opening. The results suggest that the ability of microsurgically treated human embryos to fully hatch in vitro, should be carefully (re)assessed prior to application of clinical micromanipulation systems. Micromanipulated embryos with small holes in their zonae may be rescued by performing an additional more aggressive opening procedure elsewhere on the ZP.