Removal of Retained Introns Regulates Translation in the Rapidly Developing Gametophyte of Marsilea vestita
Removal of Retained Introns Regulates Translation in the Rapidly Developing Gametophyte of Marsilea vestita
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DOI：
10.1016/j.devcel.2013.01.015
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发表时间：
2013-03-11
影响因子：
11.8
通讯作者：
Wolniak, Stephen M.
中科院分区：
文献类型：
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作者：
Boothby, Thomas C.;Zipper, Richard S.;Wolniak, Stephen M.
储存RNA的利用是快速发展的推动力。在这里，我们表明，保留和随后的删除内含子前mRNA调节时间模式的翻译过程中快速和转录后控制的精子发生的蕨类植物Marsilea vestita。RNAseq衍生的转录组的分析揭示了一个大的内含子保留转录本（IRT）的子集，编码配子发育所必需的蛋白质。基因组和IRT序列的比较表明，其他内含子已被删除以前从IRT前mRNA。完全剪接的异构体以剪接体依赖和转录独立的方式出现在发育过程中的不同时间。17/17 IRT的RNA干扰敲除在这些转录本正常剪接的时间点后产生异常。内含子保留是阻止黑木耳雄配子体转录物早熟翻译的一种功能机制。vestita。这些结果对植物基因调控具有广泛的意义，其中内含子保留是普遍存在的。
The utilization of stored RNA is a driving force in rapid development. Here, we show that retention and subsequent removal of introns from pre-mRNAs regulate temporal patterns of translation during rapid and posttranscriptionally controlled spermatogenesis of the fern Marsilea vestita. Analysis of RNAseq-derived transcriptomes revealed a large subset of intron-retaining transcripts (IRTs) that encode proteins essential for gamete development. Genomic and IRT sequence comparisons show that other introns have been previously removed from the IRT pre-mRNAs. Fully spliced isoforms appear at distinct times during development in a spliceosome-dependent and transcription-independent manner. RNA interference knockdowns of 17/17 IRTs produced anomalies after the time points when those transcripts would normally be spliced. Intron retention is a functional mechanism for forestalling precocious translation of transcripts in the male gametophyte of M. vestita. These results have broad implications for plant gene regulation, where intron retention is widespread.