Merocyanine 540 as a fluorescent probe of membranes: Selective staining of leukemic and immature hemopoietic cells
Merocyanine 540 as a fluorescent probe of membranes: Selective staining of leukemic and immature hemopoietic cells
复制标题
部花青 540 作为膜荧光探针：白血病和未成熟造血细胞的选择性染色
DOI：
10.1016/0092-8674(78)90322-7
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发表时间：
1978
期刊：
影响因子：
64.5
通讯作者：
E. Reich
中科院分区：
文献类型：
--
作者：
J. Valinsky;T. Easton;E. Reich
我们曾报道（Easton， Valinsky和Reich， 1978）， merocyanine 540 （MC 540）能特异性染色多种活的可兴奋细胞，但不能染色不可兴奋细胞。本文描述了白血病白细胞和未成熟造血前体细胞对mc540的异常通透性。我们用荧光显微镜和放射性染料摄取法研究了80例白血病患者和34例正常人外周血白细胞的mc540染色；白血病白细胞染色，而正常白细胞则不染色。白细胞染色反应不同于先前描述的可兴奋细胞的反应，因为它独立于静止介质的离子组成，动力学复杂，由光增强，由氧增强，基本上不可逆。几乎所有来自白血病个体的循环有核细胞都被染色到大致相同的程度，各种形式的白血病之间没有定性或定量的区别。
We have reported(Easton, Valinsky and Reich, 1978) that merocyanine 540 (MC 540) specifically stains a variety of living excitable cells, but not nonexcitable cells. This paper describes the exceptional permeability to MC 540 of leukemic leukocytes and immature hemopoietic precursor cells.We have used fluorescence microscopy and uptake of radioactive dye to study MC 540 staining of peripheral blood leukocytes from 80 leukemic and 34 normal individuals; leukemic leukocytes stain, whereas normal leukcytes do not. The leukocyte staining reaction differs from that previously described for excitable cells since it is independent of the ionic composition of the sta’ining medium, kinetically complex, enhanced by light, enhanced by oxygen and essentially irreversible. Virtually all circulating nucleated cells from leukemic individuals are stained to approximately the same extent, and there is no qualitative or quantitative distinction between the various forms of leukemia.