MUTANT DNAK CHAPERONES CAUSE RIBOSOME ASSEMBLY DEFECTS IN ESCHERICHIA-COLI
MUTANT DNAK CHAPERONES CAUSE RIBOSOME ASSEMBLY DEFECTS IN ESCHERICHIA-COLI
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DOI：
10.1073/pnas.90.20.9725
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发表时间：
1993-10-15
影响因子：
11.1
通讯作者：
GUERIN, MF
中科院分区：
文献类型：
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作者：
ALIX, JH;GUERIN, MF
为了确定大肠杆菌中核糖体的生物发生是否是其不同成分自组装的结果，或者涉及其他因素的参与，我们研究了分子伴侣（基因dnaK的产物）对体内核糖体组装的影响。使用三个携带突变dnaK 756-ts、dnaK 25-ts和dnaK 103-ts的热敏（ts）突变体，我们观察到在非允许温度（45 ℃）下具有不同沉降常数的核糖体颗粒的积累，即45 S、35 S和25 S沿着正常的30 S和50 S核糖体亚基。这不是热稳定性缺陷的结果，而是在非允许温度下核糖体组装缺陷的结果。这些异常的核糖体颗粒在突变细胞回到30 ℃时被拯救出来。因此，dnaK基因的产物与高温下的核糖体生物合成有关。
To determine whether the biogenesis of ribosomes in Escherichia coli is the result of the self-assembly of their different constituents or involves the participation of additional factors, we have studied the influence of a chaperone, the product of the gene dnaK, on ribosome assembly in vivo. Using three thermosensitive (ts) mutants carrying the mutations dnaK756-ts, dnaK25-ts, and dnaK103-ts, we have observed the accumulation at nonpermissive temperature (45-degrees-C) of ribosomal particles with different sedimentation constants-namely, 45S, 35S, and 25S along with the normal 30S and 50S ribosomal subunits. This is the result of a defect not in thermostability but in ribosome assembly at the nonpermissive temperature. These abnormal ribosomal particles are rescued ff the mutant cells are returned to 30-degrees-C. Thus, the product of the dnaK gene is implicated in ribosome biogenesis at high temperature.