Cell identity mediates the response of Arabidopsis roots to abiotic stress
Cell identity mediates the response of Arabidopsis roots to abiotic stress
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DOI：
10.1126/science.1153795
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发表时间：
2008-05-16
期刊：
影响因子：
56.9
通讯作者：
Benfey, Philip N.
中科院分区：
文献类型：
--
作者：
Dinneny, Jose R.;Long, Terri A.;Benfey, Philip N.
关于发育线索影响细胞如何解释环境的方式，人们知之甚少。我们的特点是转录响应高盐度的不同细胞层和发育阶段的拟南芥根，发现转录反应受到高度限制的发展参数。这些转录变化导致细胞层亚群中特定生物学功能的差异调节，其中一些对应于可观察到的生理变化。我们发现，已知的应激途径主要控制半普遍存在的反应，并使用突变体破坏表皮图案，以揭示细胞层特异性和细胞层间的影响。通过使用铁剥夺进行类似的分析，我们确定了常见的细胞类型特异性应激反应，并揭示了环境在定义细胞命运决定的转录结果中发挥的关键作用。
Little is known about the way developmental cues affect how cells interpret their environment. We characterized the transcriptional response to high salinity of different cell layers and developmental stages of the Arabidopsis root and found that transcriptional responses are highly constrained by developmental parameters. These transcriptional changes lead to the differential regulation of specific biological functions in subsets of cell layers, several of which correspond to observable physiological changes. We showed that known stress pathways primarily control semiubiquitous responses and used mutants that disrupt epidermal patterning to reveal cell-layer-specific and inter-cell-layer effects. By performing a similar analysis using iron deprivation, we identified common cell-type-specific stress responses and revealed the crucial role the environment plays in defining the transcriptional outcome of cell-fate decisions.