THE SPLICEOSOMAL SNRNAS OF CAENORHABDITIS-ELEGANS
THE SPLICEOSOMAL SNRNAS OF CAENORHABDITIS-ELEGANS
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DOI：
10.1093/nar/18.9.2633
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发表时间：
1990-05-11
影响因子：
14.9
通讯作者：
BLUMENTHAL, T
中科院分区：
文献类型：
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作者：
THOMAS, J;LEA, K;BLUMENTHAL, T
线虫是唯一已知同时发生核 mRNA 顺式和反式剪接的生物体。大多数秀丽隐杆线虫内含子都非常短，通常只有 50 个碱基长。在其他动物中发现的供体和受体剪接位点序列的结果可用于顺式和反式剪接。为了确定这些剪接事件所需的机制，我们对线虫 snRNA 进行了表征。它们在序列和结构上与其他生物体中的特征相似，在线虫 snRNA 中发现的几种序列变异为之前提出的结构模型提供了支持。线虫 snRNA 由基因家族编码。我们在这里报告了许多这些基因的序列。我们发现了一个高度保守的序列，即近端序列元件（PSE），位于迄今为止测序的所有 21 个 snRNA 基因上游约 65 bp 处，包括 SL RNA 基因，它指定了在反式剪接中提供 5'' 外显子的 snRNA。线虫 PSE 的序列与其他生物体的 PSE 序列不同。
Nematodes are the only group of organisms in which both cis- and trans-splicing of nuclear mRNAs are known to occur. Most Caenorhabditis elegans introns are exceptionally short, often only 50 bases long. The consequences donor and acceptor splice site sequences found in other animals are used for both cis- and trans-splicing. In order to identify the machinery required for these splicing events, we have characterized the C. elegans snRNAs. They are similar in sequence and structure to those characterized in other organisms, and several sequence variations discovered in the nematode snRNAs provide support for previously proposed structure models. The C. elegans snRNAs are encoded by gene families. We report here the sequences of many of these genes. We find a highly conserved sequence, the proximal sequence element (PSE), about 65 bp upstream of all 21 snRNA genes thus far sequenced, including the SL RNA genes, which specify the snRNAs that provide the 5'' exons in trans-splicing. The sequence of the C. elegans PSE is distinct from PSE''s from other organisms.