One-year stability of resin-dentin bonds created with a hydrophobic ethanol-wet bonding technique
One-year stability of resin-dentin bonds created with a hydrophobic ethanol-wet bonding technique
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DOI：
10.1016/j.dental.2009.12.009
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发表时间：
2010-04-01
期刊：
影响因子：
5
通讯作者：
Tay, Franklin R.
中科院分区：
文献类型：
--
作者：
Sadek, Fernanda T.;Castellan, Carina S.;Tay, Franklin R.
使用乙醇湿法粘合与疏水性树脂进行的牙本质粘合应该不易降解，但这一假设从未得到验证。 目的。这项体外研究评估了在人工唾液中加速老化一年后，使用实验性三步 BisGMA/TEGDMA 疏水性粘合剂或三步亲水性粘合剂创建的树脂-牙本质粘合的稳定性。从 45 颗健全的人类磨牙中获得中冠牙本质的平坦表面，并随机分为三组 (n = 15)：实验性三步 BisGMA/TEGDMA 疏水性粘合剂应用于乙醇（乙醇湿式粘合-GI）或水饱和牙本质（水湿式粘合-GII），以及根据制造商说明应用于水饱和牙本质的 Adper Scotchbond 多用途 [MP-GIII]。树脂复合牙冠逐渐成型并光固化至高度约 5 毫米。将粘合的标本在37℃的人工唾液中保存24小时并切成棒。立即和一年后对它们进行微拉伸粘合测试和 TEM 分析。使用双向方差分析和 Tukey 检验对数据进行分析。结果。老化后，MP 的微拉伸粘合强度显着降低（24 小时：40.6 +/- 2.5(a)；一年：27.5 +/- 3.3(b)；单位为 MPa）。在 TEM 检查的所有样品中，混合层降解都很明显。采用乙醇湿法粘合的疏水性粘合剂保留了粘合强度（24 小时：43.7 +/- 7.4(a)；一年：39.8 +/- 2.7(a)）和混合层完整性，后者表现出完整的胶原纤维和宽的纤维间空间。使用乙醇湿法粘合将疏水性树脂引入酸蚀牙本质中可以保持树脂-牙本质粘合完整性，无需辅助使用 MMP 抑制剂，并保证进一步的生物相容性和患者安全研究和临床测试。 (C) 2009 年牙科材料学会。由爱思唯尔有限公司出版。保留所有权利。
Dentin bonding performed with hydrophobic resins using ethanol-wet bonding should be less susceptible to degradation but this hypothesis has never been validated.Objectives. This in vitro study evaluated stability of resin-dentin bonds created with an experimental three-step BisGMA/TEGDMA hydrophobic adhesive or a three-step hydrophilic adhesive after one year of accelerated aging in artificial saliva.Methods. Flat surfaces in mid-coronal dentin were obtained from 45 sound human molars and randomly divided into three groups (n = 15): an experimental three-step BisGMA/TEGDMA hydrophobic adhesive applied to ethanol (ethanol-wet bonding-GI) or water-saturated dentin (water-wet bonding-GII) and Adper Scotchbond Multi-Purpose [MP-GIII] applied, according to manufacturer instructions, to water-saturated dentin. Resin composite crowns were incrementally formed and light-cured to approximately 5 mm in height. Bonded specimens were stored in artificial saliva at 37 degrees C for 24h and sectioned into sticks. They were subjected to microtensile bond test and TEM analysis immediately and after one year. Data were analyzed with two-way ANOVA and Tukey tests.Results. MP exhibited significant reduction in microtensile bond strength after aging (24 h: 40.6 +/- 2.5(a); one year: 27.5 +/- 3.3(b); in MPa). Hybrid layer degradation was evident in all specimens examined by TEM. The hydrophobic adhesive with ethanol-wet bonding preserved bond strength (24 h: 43.7 +/- 7.4(a); one year: 39.8 +/- 2.7(a)) and hybrid layer integrity, with the latter demonstrating intact collagen fibrils and wide interfibrillar spaces.Significance. Coaxing hydrophobic resins into acid-etched dentin using ethanol-wet bonding preserves resin-dentin bond integrity without the adjunctive use of MMPs inhibitors and warrants further biocompatibility and patient safety's studies and clinical testing. (C) 2009 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.