Ventilatory behavior and carotid body morphology of Brown Norway and Sprague Dawley rats.
Ventilatory behavior and carotid body morphology of Brown Norway and Sprague Dawley rats.
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DOI：
10.1016/j.resp.2011.06.018
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发表时间：
2011-09-15
影响因子：
2.3
通讯作者：
Strohl KP
中科院分区：
文献类型：
--
作者：
Donovan LM;Chai S;Gillombardo CB;Emancipator SN;Strohl KP
Differences in acute ventilatory behavior are associated with carotid body (CB) structural and immunohistologic profiles in some, but not all, reports. Brown Norway (BN) rats exhibit lower acute ventilatory responses to hypoxia and hypercapnia compared to Sprague Dawley (SD) rats. We hypothesized that BN rats possess CB with fewer glomus cells. Ventilation was recorded in six-month-old BN and SD rats exposed to hypoxia-reoxygenation and hypercapnia. Extracted CBs were examined using H&E staining, and immunohistochemistry with antibodies specific for Tyrosine Hydroxylase (TH), Neural Nitric Oxide Synthase (nNOS), and Pyruvate Dehydrogenase (PD). Sections were analyzed for cell and immunostaining density. SD displayed greater hypoxic and hypercapnic responses, and post-hypoxic short term potentiation, whereas BN exhibited post-hypoxic frequency decline. Contrary to our hypothesis, BN demonstrated a denser arrangement of glomus cells with a larger TH stained area (31.7% BN, 22.6% SD; p <0.0001), and nNOS stained area (37.3%BN, 32.1%; SD; p=0.01). Hence, respiratory phenotype does not correlate intuitively with these anatomic features.
