Molecular basis for the involvement of thymidine phosphorylase in cancer invasion.
Molecular basis for the involvement of thymidine phosphorylase in cancer invasion.
复制标题
DOI：
10.3892/ijmm.17.6.1085
复制
发表时间：
2006-06
影响因子：
5.4
通讯作者：
T. Gotanda;M. Haraguchi;T. Tachiwada;R. Shinkura;C. Koriyama;S. Akiba;M. Kawahara;K. Nishiyama;T. Sumizawa;T. Furukawa;H. Mimata;Y. Nomura;S. Akiyama;M. Nakagawa
中科院分区：
文献类型：
--
作者：
T. Gotanda;M. Haraguchi;T. Tachiwada;R. Shinkura;C. Koriyama;S. Akiba;M. Kawahara;K. Nishiyama;T. Sumizawa;T. Furukawa;H. Mimata;Y. Nomura;S. Akiyama;M. Nakagawa
胸苷磷酸化酶（TP），也称为血小板衍生的内皮细胞生长因子（PD-ECGF），与膀胱癌血管生成和侵袭有关。然而，其在入侵中的作用的分子基础仍不清楚。我们采用实时两步RT-PCR方法检测了72例膀胱癌组织中TP和10个侵袭相关基因的表达。我们发现TP、MMP-9、uPA和MMP-2在浸润性肿瘤中的表达水平显著高于浅表性肿瘤。TP与uPA、MMP-1、MMP-9、派-1和VEGF的表达水平呈显著正相关。高表达TP的膀胱癌细胞KK 47/TP在低氧条件下MMP-7和MMP-9的表达高于低表达TP的细胞KK/CV。PC/TP细胞，过度表达TP的前列腺癌细胞，也有更高的表达MMP-1和MMP-7比PC/CV细胞，表达没有检测到TP。综上所述，这些数据表明TP通过诱导侵袭相关基因增强肿瘤细胞的侵袭。
Thymidine phosphorylase (TP), also known as platelet-derived endothelial cell growth factor (PD-ECGF), has been implicated in bladder cancer angiogenesis and invasion. However, the molecular basis of its role in invasion remains unclear. We investigated the expression of TP and 10 invasion-related genes in bladder cancers from 72 randomly selected patients by real-time two-step RT-PCR assay. We found that the expression levels of TP, MMP-9, uPA, and MMP-2 were significantly higher in invasive tumors than those in superficial tumors. Also, the expression level of TP significantly correlated with that of uPA, MMP-1, MMP-9, PAI-1 and VEGF. KK47/TP cells, bladder cancer cells that overexpress TP, had a higher expression of MMP-7 and MMP-9 than KK/CV cells that express lower level of TP in hypoxic condition. PC/TP cells, prostate cancer cells that overexpress TP, also had a higher expression of MMP-1 and MMP-7 than PC/CV cells that express no detectable TP. Taken together these data indicate that TP enhances the invasion of tumor cells through the induction of invasion-related genes.