Controlling access time to a high-fat diet during the inactive period protects against obesity in mice
Controlling access time to a high-fat diet during the inactive period protects against obesity in mice
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DOI：
10.3109/07420528.2014.931413
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发表时间：
2014-10-01
影响因子：
2.8
通讯作者：
Shibata, Shigenobu
中科院分区：
文献类型：
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作者：
Haraguchi, Atsushi;Aoki, Natsumi;Shibata, Shigenobu
高脂饮食（HFD）的自由喂养（FF）会导致体重过度增加，而HFD的限制喂养（RF）会减弱体重增加。尚未研究用HFD喂食的时间（白天与夜晚）和喂食持续时间对能量稳态的影响。在这项研究中，我们给小鼠喂食HFD或正常饮食（ND），每天两次，在他们的活动和非活动期间，按照时间表。食物的量由喂食持续时间（2，4或8小时）调节。首先，我们研究了4小时RF在活动-非活动期（ND-ND，HFD-HFD，ND-HFD或HFD-ND）的影响。在所有4小时RF组中，小鼠消耗的卡路里几乎与FF[ ND]组相同，即使是那些喂食HFD的小鼠。体重和内脏脂肪在这三个组中低于FF[ HFD]组。其次，我们研究了RF持续时间的影响。体重和内脏脂肪在8小时组高于4小时组。体重和内脏脂肪在2小时组高于4小时组，即使2小时组有较少的食物。第三，我们研究了在非活动期吃HFD的影响，当RF持续时间延长（2，6或12小时）。在非活动期用HFD喂养小鼠2 h，在活动期用ND喂养小鼠2、6或12 h。这些小鼠的体重和内脏脂肪与FF[ ND]小鼠相当。我们的第一组实验的结果表明，4小时RF是一个足够的喂养时间来控制HFD对肥胖的影响。我们的第二组实验的结果表明，2小时RF（如快速进食）和8小时RF，代表饮食失调，是与肥胖有关的不健康的喂养模式。我们第三组实验的结果表明，在夜间短时间内吃HFD不会影响体重和内脏脂肪。总之，这些结果表明，考虑在非活动期用HFD喂养和限制饮食习惯可减轻体重增加和内脏脂肪积累的风险。
Free feeding (FF) with a high fat diet (HFD) causes excessive body weight gain, whereas restricted feeding (RF) with a HFD attenuates body weight gain. The effects of timing of feeding with a HFD (day vs. night) and feeding duration on energy homeostasis have not yet been investigated. In this study, we fed mice a HFD or a normal diet (ND) twice a day, during their active and inactive periods, on a schedule. The amount of food was regulated by feeding duration (2, 4 or 8 h). First, we investigated the effects of 4-h RF during active-inactive periods (ND-ND, HFD-HFD, ND-HFD or HFD-ND). Among all the 4-h RF groups, mice consumed almost the same amount of calories as those in the FF[ ND] group, even those fed a HFD. Body weight and visceral fat in these three groups were lower than that in the FF[ HFD] group. Second, we investigated the effects of RF duration. Body weight and visceral fat were higher in the 8-h groups than in the 4-h groups. Body weight and visceral fat were higher in the 2-h groups than in the 4-h groups even though the 2-h groups had less food. Third, we investigated the effects of eating a HFD during the inactive period, when RF duration was extended (2, 6 or 12 h). Mice were fed with a HFD during the inactive period for 2 h and fed with a ND during the active period for 2, 6 or 12 h. Body weight and visceral fat in these mice were comparable to those in the FF[ ND] mice. The results of our first set of experiments suggest that 4-h RF was an adequate feeding duration to control the effect of a HFD on obesity. The results of our second set of experiments suggest 2-h RF (such as speed-eating) and 8-h RF, representative of eating disorders, are unhealthy feeding patterns related to obesity. The results of our third set of experiments suggest that eating a HFD for a short period during the night does not affect body weight and visceral fat. Taken together, these results indicate that consideration to feeding with a HFD during the inactive period and restricting eating habits relieve the risks of body weight gain and visceral fat accumulation.