Multiple Neuropeptides in the Drosophila Antennal Lobe Suggest Complex Modulatory Circuits
Multiple Neuropeptides in the Drosophila Antennal Lobe Suggest Complex Modulatory Circuits
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DOI：
10.1002/cne.22405
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发表时间：
2010-08-15
影响因子：
2.5
通讯作者：
Nassel, Dick R.
中科院分区：
文献类型：
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作者：
Carlsson, Mikael A.;Diesner, Max;Nassel, Dick R.
果蝇（Drosophila）在觅食和繁殖过程中依赖其嗅觉。气味信息的处理发生在触角叶，这是昆虫大脑中的初级嗅觉中枢。除了经典的神经递质外，早期研究已表明嗅觉系统中存在一些神经肽。在本研究中，我们通过基质辅助激光解吸/电离 - 飞行时间（MALDI - TOF）质谱法和免疫细胞化学直接分析，对果蝇触角叶中神经肽的表达进行了广泛分析。来自7种不同前体基因的神经肽被明确鉴定，并且随后通过免疫细胞化学揭示了它们在神经元中的定位。这些神经肽包括短神经肽F、速激肽相关肽、咽侧体抑制素A、肌抑制肽、SIF酰胺、IPN酰胺和肌抑制素。神经肽在嗅觉感觉细胞的亚群以及不同的局部中间神经元和外在（离心）神经元群体中表达。在一些神经元类型中，神经肽与经典神经递质共定位。我们的研究结果表明触角叶不同回路中的肽能信号传导极为复杂。《比较神经学杂志》2010年518卷3359 - 3380页。（C）2010威利 - 利斯公司。
The fruitfly, Drosophila, is dependent on its olfactory sense in food search and reproduction. Processing of odorant information takes place in the antennal lobes, the primary olfactory center in the insect brain. Besides classical neurotransmitters, earlier studies have indicated the presence of a few neuropeptides in the olfactory system. In the present study we made an extensive analysis of the expression of neuropeptides in the Drosophila antennal lobes by direct profiling using matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) mass spectrometry and immunocytochemistry. Neuropeptides from seven different precursor genes were unambiguously identified and their localization in neurons was subsequently revealed by immunocytochemistry. These were short neuropeptide F, tachykinin related peptide, allatostatin A, myoinhibitory peptide, SIFamide, IPNamide, and myosuppressin. The neuropeptides were expressed in subsets of olfactory sensory cells and different populations of local interneurons and extrinsic (centrifugal) neurons. In some neuron types neuropeptides were colocalized with classical neurotransmitters. Our findings suggest a huge complexity in peptidergic signaling in different circuits of the antennal lobe. J. Comp. Neurol. 518:3359-3380, 2010. (C) 2010 Wiley-Liss, Inc.