Removal of the vomeronasal organ blocks the stress-induced hyperthermia response to alarm pheromone in male rats
Removal of the vomeronasal organ blocks the stress-induced hyperthermia response to alarm pheromone in male rats
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DOI：
10.1093/chemse/bjl036
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发表时间：
2007-01-01
期刊：
影响因子：
3.5
通讯作者：
Mori, Yuji
中科院分区：
文献类型：
--
作者：
Kiyokawa, Yasushi;Kikusui, Takefumi;Mori, Yuji
以前，我们报道了雄性Wistar大鼠从其肛周区域释放警报信息素，这加剧了信息素受体大鼠的应激诱导的体温过高（SIH）。随后发现这种信息素可以被困在水中，使我们能够将受体暴露在它们的笼子里。尽管它对自主神经和行为功能有明显的影响，但我们仍然没有明确的证据表明报警信息素是由主嗅觉系统（MOS）还是犁鼻系统感知的。在这项研究中，我们调查了这个问题，暴露3种类型的收件人报警信息素在他们的家笼：完整的男性（完整），犁鼻器官切除的男性（VNX），和假手术的男性（假）。完整的和假的收件人显示加重SIH响应报警信息素，而VNX收件人没有。此外，习惯化/disabituation测试和大豆凝集素结合到副嗅球的结果验证了犁鼻器（VNO）的功能MOS在信息素受体的完全消融。这些结果强烈表明，雄性大鼠感知报警信息素与VNO。
Previously, we reported that male Wistar rats release alarm pheromone from their perianal region, which aggravates stress-induced hyperthermia (SIH) in pheromone-recipient rats. The subsequent discovery that this pheromone could be trapped in water enabled us to expose recipients to the pheromone in their home cages. Despite its apparent influence on autonomic and behavioral functions, we still had no clear evidence as to whether the alarm pheromone was perceived by the main olfactory system (MOS) or by the vomeronasal system. In this study, we investigated this question by exposing 3 types of recipients to alarm pheromone in their home cages: intact males (Intact), vomeronasal organ-excised males (VNX), and sham-operated males (Sham). The Intact and Sham recipients showed aggravated SIH in response to alarm pheromone, whereas the VNX recipients did not. In addition, the results of the habituation/dishabituation test and soybean agglutinin binding to the accessory olfactory bulb verified the complete ablation of the vomeronasal organ (VNO) with a functional MOS in the pheromone recipients. These results strongly suggest that male rats perceive alarm pheromone with the VNO.