IN YEAST, RAS PROTEINS ARE CONTROLLING ELEMENTS OF ADENYLATE-CYCLASE
IN YEAST, RAS PROTEINS ARE CONTROLLING ELEMENTS OF ADENYLATE-CYCLASE
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DOI：
10.1016/0092-8674(85)90305-8
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发表时间：
1985-01-01
期刊：
影响因子：
64.5
通讯作者：
WIGLER, M
中科院分区：
文献类型：
--
作者：
TODA, T;UNO, I;WIGLER, M
含有RAS 2 val 19（具有类似于激活哺乳动物ras基因的转化潜力的错义突变的RAS 2基因）的酿酒酵母菌株具有与携带IAC或bcy 1的酵母菌株惊人相似的生长和生化特性。携带IAC突变的酵母菌株具有升高水平的腺苷酸环化酶活性。bcy 1是抑制腺苷酸环化酶缺陷型酵母中致死性的突变。RAS功能缺陷的酵母菌株表现出与腺苷酸环化酶缺陷酵母相似的性质。BCY 1抑制Ras 1-Ras 2-酵母中的致死性。与野生型酵母菌株相比，细胞内cAMP水平在RAS 2 val 19菌株中显著升高，在ras 2菌株中显著降低，并且在ras 1-ras 2-bcy 1菌株中几乎检测不到。来自ras 1-ras 2-bcy 1酵母的膜缺乏存在于来自野生型细胞的膜中的GTP刺激的腺苷酸环化酶活性，并且来自RAS 2 val 19酵母菌株的膜具有明显的GTP非依赖性腺苷酸环化酶活性的升高水平。将来自ras 1-ras 2-酵母的膜与来自腺苷酸环化酶缺陷酵母的膜混合，重建GTP依赖性腺苷酸环化酶。
Saccharomyces cerevisiae strains containing RAS2val19, a RAS2 gene with a missense mutation analogous to one that activates the transforming potential of mammalian ras genes, have growth and biochemical properties strikingly similar to yeast strains carrying IAC or bcy1. Yeast strains carrying the IAC mutation have elevated levels of adenylate cyclase activity. bcy1 is a mutation that suppresses the lethality in adenylate cyclase deficient yeast. Yeast strains deficient in RAS function exhibit properties similar to adenylate cyclase deficient yeast. bcy1 suppresses lethality in ras1- ras2- yeast. Compared to wild-type yeast strains, intracellular cAMP levels are significantly elevated in RAS2val19 strains, significantly depressed in ras2- strains, and virtually undetectable in ras1- ras2- bcy1 strains. Membranes from ras1- ras2- bcy1 yeast lack the GTP-stimulated adenylate cyclase activity present in membranes from wild-type cells, and membranes from RAS2val19 yeast strains have elevated levels of an apparently GTP-independent adenylate cyclase activity. Mixing membranes from ras1- ras2- yeast with membranes from adenylate cyclase deficient yeast reconstitutes a GTP-dependent adenylate cyclase.