Modulation of mouse preimplantation development by epidermal growth factor receptor antibodies, antisense RNA, and deoxyoligonucleotides.
Modulation of mouse preimplantation development by epidermal growth factor receptor antibodies, antisense RNA, and deoxyoligonucleotides.
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表皮生长因子受体抗体、反义 RNA 和脱氧寡核苷酸对小鼠植入前发育的调节。
DOI：
10.1002/dvg.1020140304
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Wiley,LM
中科院分区：
文献类型：
--
作者：
Brice,EC;Wu,JX;Muraro,R;Adamson,ED;Wiley,LM
将双细胞小鼠植入前胚胎在四种不同的试剂中培养48小时，以调节表皮生长因子（EGF）受体功能。这些是EGF受体、EGF受体反义RNA和EGF受体反义脱氧寡核苷酸的兔多克隆抗体和小鼠单克隆抗体。对两个终点的胚胎进行评分：作为滋养外胚层分化指标的空化发生和作为细胞增殖指标的平均胚胎细胞数。一致的观察结果是，空化显着加速抗体和反义RNA和反义脱氧寡核苷酸延迟。除多克隆抗体外，这些试剂均未对平均胚胎细胞数产生显著影响。我们对这些观察结果的解释是，抗体结合通过模拟天然配体-受体结合并诱导EGF受体典型的信号转导级联来促进空化。在反义RNA或脱氧寡核苷酸的情况下，我们建议，他们通过干扰表皮生长因子受体的生产延迟空化的发生。我们推测，在这一时期的发展，EGF受体主要是与分化的调节比与细胞增殖。© 1993 Wiley利斯公司
Two‐cell mouse preimplantation embryos were cultured for 48 h in four different reagents to modulate epidermal growth factor (EGF) receptor function. These were rabbit polyclonal and mouse monoclonal antibodies to EGF receptor, EGF receptor antisense RNA, and EGF receptor antisense deoxyoligonucleotides. Embryos were scored for two endpoints: onset of cavitation as a measure of trophectoderm differentiation and mean embryo cell number as a measure of cell proliferation. The consistent observations were that cavitation was significantly accelerated by antibodies and delayed by antisense RNA and antisense deoxyoligonucleotides. None of these reagents exerted a significant effect on mean embryo cell number, with one exception the polyclonal antibody. Our interpretation of these observations is that the antibody binding facilitated cavitation by mimicking natural ligand‐receptor binding and inducing the signal transduction cascade that is typical for the EGF receptor. In the case of antisense RNA or deoxyoligonucleotide, we propose that they delayed onset of cavitation by interfering with EGF receptor production. We hypothesize that during this period of development, EGF receptor is concerned predominantly with the regulation of differentiation more than with cell proliferation. © 1993Wiley‐Liss, Inc.