REGULATION OF CORNEAL COLLAGENASE PRODUCTION - EPITHELIAL-STROMAL CELL-INTERACTIONS
REGULATION OF CORNEAL COLLAGENASE PRODUCTION - EPITHELIAL-STROMAL CELL-INTERACTIONS
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DOI：
10.1073/pnas.75.9.4417
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发表时间：
1978-01-01
影响因子：
11.1
通讯作者：
GROSS, J
中科院分区：
文献类型：
--
作者：
JOHNSONMULLER, B;GROSS, J
从正常成年兔角膜中分离的上皮细胞和基质细胞的混合物，当与细胞松弛素B共培养时，产生潜伏的胶原酶，细胞类型单独或不含该剂的混合物都不能产生潜伏的胶原酶。该酶是一种特有的动物胶原酶，需要蛋白水解激活。上皮细胞和基质细胞的相对浓度对胶原酶的产生有深远的影响，酶活性与基质细胞的数量成正比，而与上皮细胞的数量成反比。释放到培养基中的酶的量也与细胞松弛素B的浓度成正比。经细胞松弛素b处理的上皮细胞或基质细胞的培养基不刺激其他细胞类型的胶原酶分泌。直接的细胞接触或近距离接触提示了产生性相互作用的模式，并初步确定基质细胞是酶的来源，上皮细胞是刺激物。
Mixtures of epithelial and stromal cells isolated from normal adult rabbit cornea, when cocultured with cytochalasin B, produced latent collagenase, neither cell type alone, nor the mixture without this agent, did so. The enzyme, a characteristic animal collagenase, required proteolytic activation. The relative concentrations of epithelial and stromal cells had a profound effect on collagenase production, the enzyme activity directly proportional to the number of stromal cells but inversely proportional to the number of epithelial cells. The amount of enzyme released into the medium was also directly proportional to cytochalasin B concentration. Media conditioned by cytochalasin B-treated epithelial or stromal cells did not stimulate collagenase secretion by the other cell type. Direct cell contact or close proximity suggest the mode of productive interaction and tentatively identify the stromal cell as the source of enzyme and the epithelial cell as a stimulator.