Expression of myelin proteolipid and basic protein mRNAs in cultured cells.
Expression of myelin proteolipid and basic protein mRNAs in cultured cells.
复制标题
培养细胞中髓磷脂蛋白脂质和碱性蛋白 mRNA 的表达。
DOI：
10.1002/jnr.490160118
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发表时间：
1986
影响因子：
4.2
通讯作者：
Deininger,PL
中科院分区：
文献类型：
--
作者：
Macklin,WB;Weill,CL;Deininger,PL
研究人员对培养细胞中髓磷脂特异性mRNA表达的调控进行了研究。研究了三种实验系统：作为富集细胞群生长的原代少突胶质细胞，在鸡脊髓神经元存在下生长的原代少突胶质细胞和c6细胞。采用髓鞘蛋白脂mRNA和髓鞘碱性蛋白mRNA特异性cDNA探针，定量测定不同实验条件下细胞内的蛋白脂和髓鞘碱性蛋白mRNA水平。c6细胞表达的蛋白脂mRNA少于原代少突胶质细胞表达的0.2%。原代少突胶质细胞在培养中表达髓磷脂特异性mrna至少104天，通过将大鼠少突胶质细胞与鸡脊髓神经元共培养，培养物中这些mrna的水平提高了4倍。
Studies were undertaken to investigate the regulation of myelin‐specific mRNA expression in cultured cells. Three experimental systems were investigated: primary oligodendrocytes grown as enriched cell populations, primary oligodendrocytes grown in the presence of chick spinal cord neurons, and C6cells. cDNA probes specific for the myelin proteolipid mRNA and the myelin basic protein mRNA were used to quantitate proteolipid and myelin basic protein mRNA levels in cells under different experimental conditions. C6cells expressed less than 0.2% of the proteolipid mRNA that was expressed in primary oligodendrocytes. Primary oligodendrocytes expressed the myelin‐specific mRNAs for at least 104 days in culture, and the level of these mRNAs in cultures was elevated fourfold by coculturing rat oligodendrocytes with chick spinal cord neurons.