GENETIC-CHARACTERIZATION OF COLLETOTRICHUM-GLOEOSPORIOIDES ISOLATES OBTAINED FROM MANGO
GENETIC-CHARACTERIZATION OF COLLETOTRICHUM-GLOEOSPORIOIDES ISOLATES OBTAINED FROM MANGO
复制标题
DOI：
10.1080/09670879409371887
复制
发表时间：
1994-04-01
影响因子：
1.5
通讯作者：
SREENIVASAPRASAD, S
中科院分区：
文献类型：
--
作者：
ALAHAKOON, PW;BROWN, AE;SREENIVASAPRASAD, S
从斯里兰卡不同地区的芒果叶片、花序和果实炭疽病病斑上获得的炭疽菌（Colletotrichumgloeosporioides）绝大多数菌株（> 80%）的核糖体DNA（rDNA）和线粒体DNA（mtDNA）经EcoR 1酶切后显示出相同的限制性片段带型。 此rDNA限制性片段带型与C.从其他热带国家的芒果中分离的胶孢子虫。 从花序中获得的菌株都表现出相同的rDNA和mtDNA限制性模式，但少数菌株的果实和叶片生长在混合果园芒果在斯里兰卡与两个季风和全年降雨的地区与其他水果作物的菌株的rDNA限制性模式。 这表明芒果上发生了一些交叉感染。 然而，有人建议，有一个菌株的C。gloeosporioides是芒果特有的，该菌株的克隆性质意味着它已从单一来源传播到世界各地。
Most isolates of Colletotrichum gloeosporioides (> 80%) obtained from anthracnose lesions on mango leaves, inflorescences and fruits from different regions in Sri Lanka showed the same ribosomal DNA (rDNA) and very similar mitochondrial DNA (mtDNA) restriction fragment banding patterns when genomic DNA was digested with endonuclease EcoR l. This rDNA restriction fragment banding pattern was the same as that revealed by C. gloeosporiodies isolates from mango in other tropical countries. Isolates obtained from inflorescences all showed the same rDNA and mtDNA restriction patterns but a few isolates from fruits and leaves from mango grown in mixed orchards in areas of Sri Lanka with two monsoons and year-round rainfall had rDNA restriction patterns associated with isolates from other fruit crops. This suggested that some cross-infection onto mango occurs. However, it is suggested that there is a strain of C. gloeosporioides specific to mango and the clonal nature of this strain implies that it has disseminated worldwide from a single source.