Quantification of soy isoflavones, genistein and daidzein, and conjugates in rat blood using LC/ES-MS
Quantification of soy isoflavones, genistein and daidzein, and conjugates in rat blood using LC/ES-MS
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DOI：
10.1021/jf9902651
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发表时间：
1999-09-01
影响因子：
6.1
通讯作者：
Doerge, DR
中科院分区：
文献类型：
--
作者：
Holder, CL;Churchwell, MI;Doerge, DR
染料木素是一种主要的大豆异黄酮，人们认为食用大豆有益；然而，也有人提出了不良生物效应（如雌激素、抗甲状腺）的可能性。本文建立了一种简便、灵敏的分析方法，用于测定饲用染料木素（90%）大鼠血液中染料木素的含量，在所有剂量水平下，只观察到少量的硫酸盐偶联物和苷元。没有获得额外代谢物的证据。采用电喷雾质谱和H-1核磁共振鉴定染料木黄酮的7′和4′-葡萄糖醛酸缀合物。总血染料木素从
Genistein is the principal soy isoflavone to which the putative beneficial effects of soy consumption have been attributed; however, the possibility of adverse biological effects (e.g., estrogenic, antithyroid) has also been raised. This paper describes development and validation of a simple and sensitive analytical method for the determination of genistein in the blood of rats receiving dietary genistein (90%), and only small amounts of the sulfate conjugate and the aglycone were observed at all dose levels. No evidence for additional metabolites was obtained. The 7- and 4'-glucuronide conjugates of genistein were identified using electrospray mass spectrometry and H-1 NMR. Total blood genistein ranged from