Psychroflexus sediminis sp nov., a mesophilic bacterium isolated from salt lake sediment in China

Psychroflexus sediminis sp nov., a mesophilic bacterium isolated from salt lake sediment in China
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Psychroflexus sediminis sp nov.,一种从中国盐湖沉积物中分离出来的嗜温细菌

DOI:
10.1099/ijs.0.003269-0
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发表时间:
2009-03-01
影响因子:
2.8
通讯作者:
Xu, Li-Hua
Xu, Li-Hua
中科院分区:
生物学3区
文献类型:
--
作者:
Chen, Yi-Guang;Cui, Xiao-Long;Xu, Li-Hua

文献摘要

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A novel Gram-negative, mesophilic, slightly halophilic, catalase- and oxidase-positive, obligately aerobic bacterium, designated strain YIM-C238(T), was isolated from a sediment sample from a salt lake in the Qaidam Basin, north-west China. Cells were non-sporulating, non-motile, straight to slightly curved rods. Coccoid bodies and filaments of varying length developed in older cultures. Growth occurred with 0.5-6% (w/v) NaCl [optimum, 2-3% (w/v) NaCl], at pH 6.0-10.0 (optimum, pH 7.0-8.0) and at 10-40 degrees C (optimum, 25- 30 degrees C). The major cellular fatty acids were anteiso-C-15:0, 3-OH iso-C-16:0 and anteiso-C-15:1 omega 10c, and menaquinone 6 was the sole respiratory quinone. Non-diffusible carotenoid pigments were produced. Flexirubin pigments were absent. The genomic DNA G+C content was 35.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YIM-C238(T) should be assigned to the genus Psychroflexus. The sequence similarities between the isolate and the type strains of members of the genus Psychroflexus were in the range 95.5-97.0%. The combination of phylogenetic analysis, DNA-DNA hybridization data, phenotypic characteristics and chemotaxonomic differences supported the view that strain YIM-C238(T) represents a novel species of the genus Psychroflexus, for which the name Psychroflexus sediminis sp. nov. is proposed. The type strain is YIM-C238(T) (=CCTCC AA 207030(T)=KCTC 22166(T)).