Male-killing Wolbachia in the butterfly Hypolimnas bolina
Male-killing Wolbachia in the butterfly Hypolimnas bolina
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DOI：
10.1111/j.0013-8703.2004.00179.x
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发表时间：
2004-07-01
影响因子：
1.9
通讯作者：
Murakami, R
中科院分区：
文献类型：
--
作者：
Mitsuhashi, W;Fukuda, H;Murakami, R
薄蛱蝶（鳞翅目：蛱蝶科）的某些系具有雌性偏性的特征。在这些系中，大多数雄性在到达中期幼虫阶段之前死亡。然而，造成这种偏见的原因尚不清楚。我们在雌性偏向的蝴蝶系和一些性别比例正常的蝴蝶系的所有个体中检测到沃尔巴克氏变形杆菌。四环素处理雌偏系的成年雌虫，其卵的孵化率（F1）和蛹的雌雄比显著增加。此外，在某些实验中，四环素对母蝶的处理显著提高了F1成虫的雌雄比。除沃尔巴克氏体外，已知的细菌性别比例扭曲者未被诊断性PCR检测到，也未被使用一般原核16S rDNA引物扩增的16S rDNA测序检测到。这些结果有力地表明，性别比例的扭曲是由于遗传的沃尔巴克氏菌杀死了雄性。利用沃尔巴克氏体特异性引物扩增的沃尔巴克氏体16S rDNA序列、细胞分裂蛋白基因（ftsZ）、分子伴侣groE基因（groE操纵子）和沃尔巴克氏体表面蛋白基因（wsp）在3个蝴蝶亚种（bolina、jacintha和philippensis）的系间以及雌性偏向系与正常系之间均无差异。
Some lines of the butterfly Hypolimnas bolina L. (Lepidoptera: Nymphalidae) are characterized by their female-biased sex ratio. In these lines, most males die before reaching the middle larval stage. However, the cause of the bias remains unclear. We detected the proteobacterium Wolbachia in all individuals in the female-biased butterfly lines and in some of the lines with a normal sex ratio. Tetracycline treatment of adult females of a female-biased line led to a significant increase in both the hatch rate of their eggs (F1) and the male-to-female ratio of F1 pupae. In addition, certain assays of tetracycline treatment on mother butterflies significantly increased the male to female ratio of F1 adults. Known bacterial sex ratio distorters other than Wolbachia were not detected by diagnostic PCR assay, nor by the sequencing of 16S rDNA amplified using general prokaryotic 16S rDNA primers. These results strongly suggest that the distortion of the sex ratio is due to the killing of males by the inherited Wolbachia. Sequences of the 16S rDNA amplified using Wolbachia-specific primers, the cell division protein gene (ftsZ), the molecular chaperone groE genes (groE operon), and the Wolbachia surface protein gene (wsp) from Wolbachia in lines belonging to three subspecies of the butterfly (bolina, jacintha, and philippensis) revealed no variation among lines nor between female-biased lines and a normal one.