Cocktails of probiotics pre-adapted to multiple stress factors are more robust under simulated gastrointestinal conditions than their parental counterparts and exhibit enhanced antagonistic capabilities against Escherichia coli and Staphylococcus aureus
Cocktails of probiotics pre-adapted to multiple stress factors are more robust under simulated gastrointestinal conditions than their parental counterparts and exhibit enhanced antagonistic capabilities against Escherichia coli and Staphylococcus aureus
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DOI：
10.1186/s13099-015-0053-5
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发表时间：
2015-02-26
期刊：
影响因子：
4.2
通讯作者：
Thantsha, Mapitsi Silvester
中科院分区：
文献类型：
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作者：
Mathipa, Moloko Gloria;Thantsha, Mapitsi Silvester
背景资料：益生菌在提供健康益处方面的成功取决于它们承受技术和胃肠道条件的能力;因此，开发强大的培养物对益生菌行业至关重要。益生菌培养物的组合已被证明比使用单一培养物更有效地治疗和预防异质性疾病。我们研究了益生菌的预适应的多重应力对它们在模拟胃肠道条件下的稳定性的影响，以及它们的单一的影响，以及它们对选定的肠道病原体的协同拮抗作用。方法：将益生菌培养物接种到MRS肉汤中，调节至pH 2，并在37 ℃下孵育2小时。将pH 2的存活细胞在37 ℃下在2%胆汁酸中传代培养1小时。最后将暴露于2%胆汁酸1小时后显示生长的细胞接种于新鲜MRS肉汤中，并在55 ℃下孵育2小时。然后将存活的细胞用作应激适应培养物。适应的文化暴露于模拟胃肠道条件和他们的非适应对应物被用来比较应激适应的影响。结果：大多数应激适应细胞的耐酸性和耐胆汁性均高于非适应细胞。连续暴露于模拟胃液和肠液后，所有适应应激的乳酸杆菌和长双歧杆菌LMG 13197的活菌计数均较高。然而，对于B。longum Bb 46和B.两歧双歧杆菌LMG 13197，暴露于这些液体后，非适应细胞的存活率高于适应细胞。一种含有L. plantarum + B.长穗型Bb 46 + B。longum LMG 13197对S. aureus，而E.大肠杆菌的抑菌效果以L.嗜酸乳杆菌La 14 150 B + B。长穗型Bb 46 + B。两歧LMG 11041。一个鸡尾酒含有六个非适应文化是最有效的抑制pathogenes.Conclusion：多应力预适应增强益生菌在模拟胃肠道条件下的生存能力;和配方含有多应力适应细胞的混合物表现出增强的协同作用，对食源性病原体。
Background: The success of the probiotics in delivery of health benefits depends on their ability to withstand the technological and gastrointestinal conditions; hence development of robust cultures is critical to the probiotic industry. Combinations of probiotic cultures have proven to be more effective than the use of single cultures for treatment and prevention of heterogeneous diseases. We investigated the effect of pre-adaptation of probiotics to multiple stresses on their stability under simulated gastrointestinal conditions and the effect of their singular as well as their synergistic antagonistic effect against selected enteric pathogens.Methods: Probiotic cultures were inoculated into MRS broth adjusted to pH 2 and incubated for 2 h at 37 degrees C. Survivors of pH 2 were subcultured into 2% bile acid for 1 h at 37 degrees C. Cells that showed growth after exposure to 2% bile acid for 1 h were finally inoculated in fresh MRS broth and incubated at 55 degrees C for 2 h. The cells surviving were then used as stress adapted cultures. The adapted cultures were exposed to simulated gastrointestinal conditions and their non-adapted counterparts were used to compare the effects of stress adaptation. The combination cultures were tested for their antipathogenic effects on Escherichia coli and Staphylococcus aureus.Results: Acid and bile tolerances of most of the stress-adapted cells were higher than of the non-adapted cells. Viable counts of all the stress-adapted lactobacilli and Bifidobacterium longum LMG 13197 were higher after sequential exposure to simulated gastric and intestinal fluids. However, for B. longum Bb46 and B. bifidum LMG 13197, viability of non-adapted cells was higher than for adapted cells after exposure to these fluids. A cocktail containing L. plantarum + B. longum Bb46 + B. longum LMG 13197 best inhibited S. aureus while E. coli was best inhibited by a combination containing L. acidophilus La14 150B + B. longum Bb46 + B. bifidum LMG 11041. A cocktail containing the six non-adapted cultures was the least effective in inhibiting the pathogens.Conclusion: Multi-stress pre-adaptation enhances viability of probiotics under simulated gastrointestinal conditions; and formulations containing a mixture of multi stress-adapted cells exhibits enhanced synergistic effects against foodborne pathogens.