PD-L1 expression is associated with epithelial-mesenchymal transition in head and neck squamous cell carcinoma.
PD-L1 expression is associated with epithelial-mesenchymal transition in head and neck squamous cell carcinoma.
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DOI：
10.18632/oncotarget.7431
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发表时间：
2016-03-29
期刊：
影响因子：
--
通讯作者：
Heo DS
中科院分区：
文献类型：
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作者：
Ock CY;Kim S;Keam B;Kim M;Kim TM;Kim JH;Jeon YK;Lee JS;Kwon SK;Hah JH;Kwon TK;Kim DW;Wu HG;Sung MW;Heo DS
病毒相关恶性肿瘤和肉瘤样癌与PD-L1高表达相关，然而，潜在的机制仍然存在争议。我们评估了PD-L1表达与头颈部鳞状细胞癌（HNSCC）上皮-间质转化（EMT）之间的相关性。通过免疫组织化学对50例HNSCC患者的肿瘤组织进行了PD-L1评价，结果显示32例（64.0%）PD-L1阳性（PD-L1+）。有趣的是，PD-L1表达与EMT显著相关（P = 0.010），如通过低E-钙粘蛋白和高波形蛋白表达评估的。具有EMT特征的PD-L1+患者的总生存率显著低于无EMT特征的患者（P = 0.007）。在独立验证队列（N = 91）以及癌症基因组图谱（TCGA）和癌细胞系百科全书的HNSCC病例中，高PD-L1表达也与EMT特征的高概率相关，参考GEO数据集GSE 4824。生存分析证实，在TCGA队列中，PD-L1 +/EMT+患者的预后比PD-L1+/EMT-患者差。因此，PD-L1阳性可以根据EMT的存在或不存在分为两类。PD-L1表达也与HNSCC中的EMT特征独立相关。
Virus-associated malignancies and sarcomatoid cancers correlate with high PD-L1 expression, however, underlying mechanisms remain controversial. We evaluated the correlation between PD-L1 expression and epithelial-mesenchymal transition (EMT) in head and neck squamous cell carcinomas (HNSCC). Tumor tissues from 50 patients with HNSCC were evaluated for PD-L1 by immunohistochemistry, which showed 32 (64.0%) were PD-L1 positive (PD-L1+). Interestingly, PD-L1 expression was significantly associated with EMT (P = 0.010), as assessed by low E-cadherin and high vimentin expression. The overall survival of PD-L1+ patients with EMT features was significantly worse than those without EMT features (P = 0.007). In an independent validation cohort (N = 91), as well as in HNSCC cases of The Cancer Genome Atlas (TCGA) and the Cancer Cell Line Encyclopedia, high PD-L1 expression was also associated with the high probability of an EMT signature, referred from the GEO dataset, GSE4824. Survival analysis confirmed PD-L1+/EMT+ patients had a poorer prognosis than PD-L1+/EMT- patients in the TCGA cohort. PD-L1 positivity can thus be divided into two categories according to the absence or presence of EMT. PD-L1 expression is also independently associated with EMT features in HNSCC.