An improved procedure for serum creatine phosphokinase determination.
An improved procedure for serum creatine phosphokinase determination.
复制标题
血清肌酸磷酸激酶测定的改进程序。
DOI：
--
复制
发表时间：
1967
期刊：
影响因子：
--
通讯作者：
S. Rosalki
中科院分区：
文献类型：
--
作者：
S. Rosalki
摘要 血清肌酸磷酸激酶活性是通过酶作用释放的三磷酸腺苷与烟酰胺腺嘌呤二核苷酸磷酸的还原以及分光光度法形成还原型烟酰胺腺嘌呤二核苷酸磷酸相关联来测定的。用于反应的酶底物批量制备、冷冻干燥并分布在明胶覆盖的胶囊中。胶囊内容物在使用前立即添加蒸馏水进行重构，并通过测量添加血清后 340 mμ 处光密度的增加来测定肌酸磷酸激酶活性。与实验室常用的血清肌酸磷酸激酶测定方法相比，该方法方便、灵敏、耗时少。
Abstract The creatine phosphokinase activity of serum is determined by a procedure in which adenosine triphosphate, liberated by the action of the enzyme, is linked to the reduction of nicotinamide-adenine dinucleotide phosphate and the formation of reduced nicotinamide-adenine dinucleotide phosphate followed spectrophotometrically. The enzyme substrate for the reaction is prepared in bulk, freeze-dried, and distributed in gelatinecovered capsules. The capsule contents are reconstituted immediately before use by the addition of distilled water, and creatine phosphokinase activity is determined by measuring the increase in optical density at 340 mμ following serum addition. The method is convenient, more sensitive, and less time-consuming than other procedures for serum creatine phosphokinase determination in common laboratory use.