Angiotensin II directly increases transforming growth factor β1 and osteopontin and indirectly affects collagen mRNA expression in the human heart
Angiotensin II directly increases transforming growth factor β1 and osteopontin and indirectly affects collagen mRNA expression in the human heart
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DOI：
10.1016/s0008-6363(00)00037-7
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发表时间：
2000-06-01
影响因子：
10.8
通讯作者：
Regitz-Zagrosek, V
中科院分区：
文献类型：
--
作者：
Kupfahl, C;Pink, D;Regitz-Zagrosek, V
目的：血管紧张素II(Ang II)可诱导心肌成纤维细胞增殖和胶原合成，但其在人心脏中的确切作用机制尚不清楚。因此，我们研究了Ang II是否直接影响人心肌和分离的人心脏成纤维细胞中胶原mRNA的含量，或者生长因子转化生长因子β-1和骨桥蛋白是否参与了这一过程。方法和结果I：在第一组实验中，采用短时间刺激法测定Ang II对新鲜人心房肌I型胶原、转化生长因子β1和骨桥蛋白mRNA含量的直接影响。4h后，Ang II刺激的心房组织中两种转化生长因子β1的表达均显著高于对照组(183+/-21%，p
Objectives: Angiotensin II (Ang II) induces fibroblast proliferation and collagen synthesis in the myocardium, but its precise mechanisms of action in human hearts are still unknown. Therefore, we investigated whether Ang II directly affects the collagen mRNA content in the human myocardium and in isolated human cardiac fibroblasts or whether the growth factors TGF beta-1 and osteopontin are involved in this process. Methods and results I: In a first set of experiments, the direct effect of Ang II on collagen I, TGF beta-1 and osteopontin mRNA content in fresh samples of human atrial myocardium was determined by the use of a short stimulation period. After 4 h, Ang II-stimulated atrial samples gave a significantly higher expression of both TGF beta-1 (183+/-21% of control, p