Cloning of the promoter region of β-xylosidase (FaXyl1) gene and effect of plant growth regulators on the expression of FaXyl1 in strawberry fruit
Cloning of the promoter region of β-xylosidase (FaXyl1) gene and effect of plant growth regulators on the expression of FaXyl1 in strawberry fruit
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DOI：
10.1016/j.plantsci.2009.03.015
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发表时间：
2009-07-01
期刊：
影响因子：
5.2
通讯作者：
Martinez, Gustavo A.
中科院分区：
文献类型：
--
作者：
Bustamante, Claudia A.;Civello, Pedro M.;Martinez, Gustavo A.
参与果实软化的细胞壁修饰酶的表达和活性可能受激素和/或其他信号分子的调节。在草莓中，FaXyl1编码一种水果特有的β-木糖苷酶，可能与半纤维素降解有关。在本工作中，我们分离并分析了FaXyl1基因的启动子区域。对序列的分析揭示了与激素、光和胁迫相关反应相关的顺式作用元件的存在。为了证明FaXyl1对植物生长调节剂的反应性，在果实上进行了几种处理，这些调节剂与启动子区域中确定的调节元件(脱落酸、生长素和赤霉素)以及与成熟过程相关的其他元件(乙烯和一氧化氮)有关。研究了不同处理对FaXyl1表达、相应蛋白水平和β-木糖苷酶活性的影响。ABA(脱落酸)刺激FaXyl1的表达和蛋白水平。相反，在NAA(萘乙酸)、GA(3)(赤霉酸)和乙烯生成化合物乙烯利处理后，FaXyl1基因的表达水平下降。NO供体硝普钠(SNP)不影响FaXyl1mRNA和蛋白水平。1-MCP(1-甲基环丙烯)对FaXyl1表达的影响与乙烯利的作用一致。β-木糖苷酶活性受NAA抑制，而GA3、ABA、乙烯利、1-MCP和SNP对其无影响。(C)2009爱思唯尔爱尔兰有限公司。保留所有权利。
Expression and activity of cell wall modifying enzymes involved in fruit softening may be regulated by hormones and/or other signal molecules. In strawberry, FaXyl1 encodes for a fruit-specific beta-xylosidase probably associated to hemicellulose degradation. In this work, we have isolated and analysed the promoter region of FaXyl1 gene. Analysis of the sequence revealed the presence of cis-acting elements associated with hormone, light and stress-related responses. Several treatments were done on fruit in order to prove the responsiveness of FaXyl1 to plant growth regulators related to the regulatory elements identified in the promoter region (abscisic acid, auxins and gibberellins) and others associated with the ripening process (ethylene and nitric oxide). The effect of each treatment on FaXyl1 expression, the corresponding protein levels and the beta-xylosidase activity was evaluated. ABA (abscisic acid) stimulated FaXyl1 expression and protein levels. In contrast, expression levels of FaXyl1 gene decreased after treatments with NAA (naphthalene acetic acid), GA(3) (gibberellic acid) and ethephon, an ethylene-generating compound. SNP (sodium nitroprusside), a NO donor, did not affect FaXyl1 mRNA and protein levels. The effect of 1-MCP (1-methylcyclopropene), an ethylene perception inhibitor, on FaXyl1 expression was consistent with the effect observed with ethephon. beta-xylosidase activity was down regulated by NAA, whereas GA3, ABA, ethephon, 1-MCP and SNP had no effect on it. (C) 2009 Elsevier Ireland Ltd. All rights reserved.