THE LYMPHOCYTE-T GLYCOPROTEIN CD2 BINDS THE CELL-SURFACE LIGAND LFA-3
THE LYMPHOCYTE-T GLYCOPROTEIN CD2 BINDS THE CELL-SURFACE LIGAND LFA-3
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DOI：
10.1038/326400a0
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发表时间：
1987-03-26
期刊：
影响因子：
64.8
通讯作者：
SPRINGER, TA
中科院分区：
文献类型：
--
作者：
SELVARAJ, P;PLUNKETT, ML;SPRINGER, TA
CD 2（也称为T11（参考文献1）、LFA-2（参考文献2）和红细胞玫瑰花结受体（参考文献3））是一种功能重要的T淋巴细胞表面糖蛋白，相对分子质量为50，000至58，0004（Mr 50 -58 K），在胸腺细胞个体发育早期出现，并存在于所有成熟T细胞上5。抗CD 2单克隆抗体通过与T淋巴细胞结合并阻断与靶细胞的粘附来抑制细胞毒性T淋巴细胞（CTL）介导的杀伤作用2 - 4，6。此类抗体还抑制T辅助细胞应答，包括抗原刺激的增殖、白细胞介素-2（IL-2）分泌和IL-2受体表达2 - 4，7 -9。CD 2表位单克隆抗体的某些组合可触发外周血T淋巴细胞增殖1、细胞毒性效应子功能10和胸腺细胞表达IL-2受体，导致外源性IL-2存在时胸腺细胞增殖（参考文献11）。这些发现表明，CD 2可以在信号传导中起作用，也可以是粘附分子。为了了解CD 2在T细胞粘附和活化中的作用，必须确定其天然配体。我们先前观察到纯化的CD 2抑制T淋巴细胞与绵羊红细胞的重置，并且可以被绵羊红细胞吸收12，这表明它也可能以可检测的亲和力与人细胞结合。我们现在报告，CD 2结合细胞表面抗原称为淋巴细胞功能相关抗原-3（LFA-3）具有高亲和力，并可以介导的粘附淋巴细胞通过与LFA-3的相互作用。
CD2 (known also as T11 (ref. 1), LFA-2 (ref. 2) and the erythrocyte rosette receptor (ref. 3)) is a functionally important T lymphocyte surface glycoprotein of relative molecular mass 50,000 to 58,0004(Mr50–58 K) which appears early in thymocyte ontogeny and is present on all mature T cells5. Monoclonal antibodies to CD2 inhibit cytotoxic T-lymphocyte (CTL)-mediated killing by binding to the T lymphocyte and blocking adhesion to the target cell2–4,6. Such antibodies also inhibit T helper cell responses including antigen-stimulated proliferation, interleukin-2 (IL-2) secretion, and IL-2 receptor expression2–4,7–9. Certain combinations of monoclonal antibodies to CD2 epitopes trigger proliferation of peripheral blood T lymphocytes1, cytotoxic effector function10and expression of IL-2 receptors by thymocytes, resulting in thymocyte proliferation in the presence of exogenous IL-2 (ref. 11). These findings suggest that CD2 can function in signalling as well as being an adhesion molecule. To understand the role of CD2 in T-cell adhesion and activation, it is essential to define its natural ligand. Our previous observation that purified CD2 inhibits resetting of T lymphocytes with sheep erythrocytes and can be absorbed by sheep erythrocytes12suggested it also might bind with detectable affinity to human cells. We now report that CD2 binds to a cell-surface antigen known as lymphocyte function-associated anti-gen-3 (LFA-3) with high affinity, and can mediate adhesion of lymphoid cells via interaction with LFA-3.