Cardiolipin enrichment in spore membranes and its involvement in germination of Bacillus subtilis Marburg
Cardiolipin enrichment in spore membranes and its involvement in germination of Bacillus subtilis Marburg
复制标题
DOI：
10.1266/ggs.81.69
复制
发表时间：
2006-04-01
影响因子：
1.1
通讯作者：
Matsumoto, Kouji
中科院分区：
文献类型：
--
作者：
Kawai, Fumitaka;Hara, Hiroshi;Matsumoto, Kouji
用二硫苏糖醇/尿素和NaOH处理孢子，然后溶菌酶消化，提取马尔堡菌，发现孢子膜的心磷脂（CL）含量显著高于指数生长细胞的膜。对不经化学处理而易被溶菌酶消化的被毛缺陷、cotE::cat和gerE::cat突变孢子的膜进行分析，证实孢子膜含有高水平的CL。在加入萌发物l -丙氨酸或AGFK（天冬酰胺、葡萄糖、果糖和KCI的组合）后，野生型孢子悬浮液的浊度在30分钟内降至50%。然而，当添加l -丙氨酸时，仅添加微量CL的孢子悬浮液的浊度没有下降，而AGFK的浊度最初下降幅度很小（60分钟后为14%）。这些结果表明，CL参与了萌发的早期阶段，与萌发受体的功能有关。这是体内第一个明显的证据，证明CL在细菌膜中具有特殊的作用，在这种作用中它不能被其他阴离子磷脂取代。
Marburg, extracted after treatment of spores with dithiothreitol/urea and NaOH followed by lysozyme digestion, revealed that the spore membranes had significantly higher cardiolipin (CL) content than the membranes of exponentially growing cells. Analysis of the membranes of coat-defective, cotE::cat and gerE::cat mutant spores, which are susceptible to lysozyme digestion without chemical treatment, confirmed that spore membranes contain a high level of CL. After addition of the germinants L-alanine or AGFK (a combination of asparagine, glucose, fructose, and KCI), the turbidity of wild type spore suspensions decreased to 50% within 30 min. Suspensions of spores with only trace amounts of CL, however, showed no decrease in turbidity when L-alanine was added and the initial decrease in turbidity with AGFK was slight (14% after 60 min). These results indicate that CL is involved in an early step of germination, related to the functioning of germinant receptors. This is the first conspicuous in vivo evidence that CL in bacterial membranes has a specific role, in which it cannot be replaced by other anionic phospholipids.