VEGF localisation in diabetic retinopathy
VEGF localisation in diabetic retinopathy
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DOI：
10.1136/bjo.82.5.561
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发表时间：
1998-05-01
影响因子：
4.1
通讯作者：
McLeod, D
中科院分区：
文献类型：
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作者：
Boulton, M;Foreman, D;McLeod, D
目的探讨血管内皮生长因子（VEGF）在糖尿病视网膜病变不同阶段的表达特点（包括激光光凝后），并比较切除的纤维血管和纤维细胞中的染色方法：VEGF的免疫组织化学定位，使用抗VEGF（165）和VEGF（121，165，189），在正常人视网膜（n=15），糖尿病视网膜（（a）无明显视网膜病变（n=19），（B）有视网膜内血管异常但无增殖性视网膜病变（n=6），（c）有活动性增殖性视网膜病变（n=6），（d）在光凝治疗后无残余增殖性视网膜病变（n=15））、切除的糖尿病纤维血管膜（n=19）和非糖尿病纤维细胞膜（n=7）。免疫染色的程度和模式recorded.Results-In一般情况下，VEGF是从大多数正常视网膜缺席。VEGF染色在大多数糖尿病组织中是明显的，但染色模式取决于所用抗体的特异性和组织的类别。VEGF（165）抗体的染色通常局限于内皮细胞和血管周围区域，而VEGF（121，165，189）抗体也与内层视网膜的血管外成分相关。糖尿病眼的免疫染色强度取决于视网膜病变的严重程度，在无视网膜病变的糖尿病患者中最低，在增殖性视网膜病变的视网膜中最高。有趣的是，在糖尿病视网膜中，经过激光手术的增生性视网膜病变的免疫染色强度降低到基础水平。中度到强烈的免疫染色观察到在所有fibrovascular和fibrocellular membranesexamined.Conclusions-This研究支持VEGF在糖尿病视网膜病变的临床前和增殖阶段的发病机制中的间接作用。
Aim-To determine the staining pattern of vascular endothelial growth factor (VEGF) at different stages of diabetic retinopathy (including post-laser photocoagulation) and to compare staining in excised fibrovascular and fibrocellular (non-diabetic) preretinal membranes.Methods-Immunohistochemical localisation of VEGF, using antibodies raised against VEGF(165) and VEGF(121,165,189), was carried out on specimens of normal human retina (n=15), diabetic retinas ((a) with no overt retinopathy (n=19), (b) with intraretinal vascular abnormalities but no proliferative retinopathy (n=6), (c) with active proliferative retinopathy (n=6), (d) with no residual proliferative retinopathy after photocoagulation therapy (n=15)), excised diabetic fibrovascular membranes (n=19), and non-diabetic fibrocellular membranes (n=7). The degree and pattern of immunostaining was recorded.Results-In general, VEGF was absent from the majority of normal retinas. VEGF staining was apparent in most diabetic tissues but the staining pattern was dependent on both the specificity of the antibody used and the category of tissue. Staining with the VEGF(165) antibody was generally confined to endothelial cells and perivascular regions while the VEGF(121,165,189) antibody was also associated with extravascular components of the inner retina. Intensity of immunostaining of diabetic eyes was dependent on the severity of retinopathy being least in diabetics with no avert retinopathy and greatest in retinas with proliferative retinopathy. Interestingly, the intensity of immunostaining in diabetic retinas which had undergone laser surgery far proliferative retinopathy was reduced to basal levels. Moderate to intense immunostaining was observed in all fibrovascular and fibrocellular membranes examined.Conclusions-This study supports at circumstantial role for VEGF in the pathogenesis of both the preclinical and proliferative stages of diabetic retinopathy.