Initial cell type divergence in Dictyostelium is independent of DIF-1
Initial cell type divergence in Dictyostelium is independent of DIF-1
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DOI：
10.1006/dbio.1996.0067
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发表时间：
1996-03-15
影响因子：
2.7
通讯作者：
Loomis, WF
中科院分区：
文献类型：
--
作者：
Shaulsky, G;Loomis, WF
前孢子和前柄细胞可以区分内聚集的网松藻的良好特征的细胞类型特异性基因的表达。tagB调控区与大肠杆菌β-半乳糖苷酶的融合揭示了这种前茎特异性基因标志着初始前茎细胞群体PST-1的分化。报告基因在tagB（-）突变细胞中正常表达，尽管它们不能积累可测量水平的DIF-1，DIF-1是一种以前与前茎分化有关的形态原。在一个独立的实验系统中，野生型细胞通过诱导前茎标记ecmA和抑制前孢子标记cotB来响应DIF-1的加入。我们发现，DIE-I不影响tagB或carB基因的表达，这两个基因都是前茎特异性的，对PST-A细胞分化至关重要。我们的结论是，启动prestalk的发展是不依赖于DIF-1，并建议形态主要参与在后期阶段。(C)1996年，Academic Press，Inc.
Prespore and prestalk cells can be distinguished within aggregates of Dictyosrelium by the expression of well-characterized cell type-specific genes. Fusion of the tagB regulatory region to Escherichia coli beta-galactosidase revealed that this prestalk-specific gene marks the differentiation of the initial prestalk cell population, PST-1. The reporter gene was expressed normally in tagB(-) mutant cells despite the fact that they do not accumulate measurable levels of DIF-1, a morphogen that was previously implicated in prestalk differentiation. In an independent experimental system, wild-type cells respond to the addition of DIF-1 by induction of the prestalk marker ecmA and repression of the prespore marker cotB. We found that DIE-I did not affect the expression of the tagB or carB genes, both of which are prestalk specific and essential for PST-A cell differentiation. We conclude that the initiation of prestalk development is not dependent on DIF-1 and suggest that the morphogen participates mainly at later stages. (C) 1996, Academic Press, Inc.