Jogyamycin, a new antiprotozoal aminocyclopentitol antibiotic, produced by Streptomyces sp a-WM-JG-16.2
Jogyamycin, a new antiprotozoal aminocyclopentitol antibiotic, produced by Streptomyces sp a-WM-JG-16.2
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DOI：
10.1038/ja.2011.136
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发表时间：
2012-03-01
影响因子：
3.3
通讯作者：
Omura, Satoshi
中科院分区：
文献类型：
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作者：
Iwatsuki, Masato;Nishihara-Tsukashima, Aki;Omura, Satoshi
在我们寻找新的抗原动物（抗疟疾和抗锥虫）剂，我们已经测试了各种来源的土壤微生物的培养提取物，包括北里生命科学研究所和生物资源实验室，Micro-Biopharm日本公司分离的那些。我们以前已经发现了多种微生物代谢产物，表现出抗原生动物，1，2抗疟疾3，4和抗锥虫的特性。最近，我们从链霉菌属a-WM-JG-16.2的培养液中分离出一种新的帕他霉素衍生物，命名为乔吉霉素（图1）。已证明该化合物具有有效的抗原生动物活性（表2）。本文报道了氨基环戊醇的发酵、分离、结构鉴定和生物活性。使用蔗糖密度梯度离心法，从印度尼西亚Jogjakartan收集的土壤样品中分离出生产生物体菌株a-WM-JG-16.2。7使用基本的局部比对搜索工具（BLAST），我们比较了EzTaxon服务器中可用的16 S rRNA基因序列以鉴定菌株a-WM-JG-16.2的种。链霉菌a-WM-JG-16.2为链霉菌属的一个种，其16 S rRNA基因序列与链霉菌属的链霉菌（S.灰红NBRC 12873将该菌株在旋转振荡器（220 rpm）上在28 ℃下在500-ml锥形瓶中培养5天（20个烧瓶），含50 ml含2%大米淀粉的生产培养基（Rose牌，Cikampek，印度尼西亚）、2%葡萄糖（Univar，Sydney，NSW，澳大利亚）、2%大豆粉（J-Oil米尔斯，东京，日本）、0.5%酵母提取物
During our search for new antiprotozoal (antimalarial and antitrypanosomal) agents, we have tested culture extracts of soil microorganisms from a variety of sources, including those isolated by the Kitasato Institute for Life Sciences and the Bioresource Laboratories, Micro-Biopharm Japan Corporation. We have previously discovered a variety of microbial metabolites exhibiting antiprotozoal, 1, 2 antimalarial 3, 4 and antitrypanosomal properties. 5, 6 Recently, we have isolated a new derivative of pactamycin, designated as jogyamycin (Figure 1), from a culture broth of Streptomyces sp. a-WM-JG-16.2. This compound has proved to have potent antiprotozoal activity (Table 2). Here, we report the fermentation, isolation, structure elucidation and biological activity of this novel aminocyclopentitol antibiotic. The producing organism, strain a-WM-JG-16.2 was isolated from a soil sample collected in Jogjakarta, Indonesia, using the sucrose density gradient centrifugation method. 7 Using the basic local alignment search tool (BLAST), we compared the 16S rRNA gene sequences available in the EzTaxon server to identify the species of strain a-WM-JG-16.2. Streptomyces sp. a-WM-JG-16.2 was classified as a species of the genus Streptomyces; the 16S rRNA gene sequence comparison showing a 100% similarity with S. griseoruber NBRC12873 (AB184209).The strain was cultured on a rotary shaker (220 rpm) at 281C for 5 days in 500-ml Erlenmeyer flasks (20 flasks) containing 50ml of a production medium containing 2% rice starch (Rose brand, Cikampek, Indonesia), 2% glucose (Univar, Sydney, NSW, Australia), 2% soybean meal (J-Oil Mills, Tokyo, Japan), 0.5% yeast extract