The effects of a high-fat and high-energy diet on the hepatic expression of CYP3A in developing female rats
The effects of a high-fat and high-energy diet on the hepatic expression of CYP3A in developing female rats
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DOI：
10.3109/00498254.2011.645907
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发表时间：
2012-05
期刊：
影响因子：
1.8
通讯作者：
Shao-qing Ni;Xiumin Wang;Jue Wang;Zhengyan Zhao;S. Zeng
中科院分区：
文献类型：
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作者：
Shao-qing Ni;Xiumin Wang;Jue Wang;Zhengyan Zhao;S. Zeng
本研究旨在探讨高脂高能饮食对发育期雌性大鼠肝脏细胞色素P-450 3A（CYP 3A）表达的影响。用标准饲料喂养的母鼠幼崽定义为NN组，用HFHE饲料喂养的母鼠幼崽定义为NH组。测定mRNA和蛋白表达、蛋白定位和活性。NH组在第3天的CYP 3A 1 mRNA表达高于NN组（p < 0.05），而NH组在第28天和第56天的表达低于NN组（p < 0.01）。CYP 3A 1免疫标记在NN组28 d及以后呈带状表达模式，而在NH组84 d观察到明显的带状表达模式。NH组在第3、7、14和28天的平均活性高于NN组（p < 0.05）。在第84天，NH组的活性低于NN组（p < 0.05）。本研究结果为进一步研究肥胖儿童的合理用药方案提供了依据。
We aimed to investigate the effects of high-fat and high-energy (HFHE) diets on the hepatic expression of cytochrome P-450 3A (CYP3A) in developing female rats. The pups of the dams fed with the standard diet were defined as the NN group and those fed the HFHE diet were defined as the NH group. The mRNA and protein expression, the protein localization and activity was determined. The mRNA expression of CYP3A1 on day 3 in the NH group were higher versus NN groups (p < 0.05) and the expression of the NH group on days 28 and 56 were lower versus the NN group (p < 0.01). CYP3A1 immunolabeling had a zonal-restricted expressions pattern on day 28 and after in the NN groups, while the obvious zonal expression pattern was observed in the NH group on day 84. The mean activity for the NH groups on days 3, 7, 14 and 28 was higher versus the NN groups (p < 0.05). On day 84, the activity was lower for the NH group versus the NN group (p < 0.05). Our findings provide a basis for further studies on appropriate medication regimen in obese children.