Short Report: Malaria Diagnosis by a Polymerase Chain Reaction-Based Assay Using a Pooling Strategy
Short Report: Malaria Diagnosis by a Polymerase Chain Reaction-Based Assay Using a Pooling Strategy
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DOI：
10.4269/ajtmh.2009.09-0274
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发表时间：
2009-11-01
影响因子：
3.3
通讯作者：
Smith, Davey M.
中科院分区：
文献类型：
--
作者：
Bharti, Ajay R.;Letendre, Scott L.;Smith, Davey M.
汇集临床标本减少了筛查传染病时所需的化验次数。基于聚合酶链反应（PCR）的检测方法是诊断疟疾最敏感的检测方法，但其高昂的成本限制了其使用。我们采用了一个汇集平台，可以减少检测疟疾感染所需的化验次数。为了评估这一平台，对两组1.00份血清样本进行了检测，分别为1%和5%的疟疾流行率。从混合样本中提取的DNA通过疟疾特异性PCR扩增。在1:10和1:10稀释的基础上，通过检测PCR检测水平进行进一步验证。该平台正确地检测了两种测试基质中的所有疟疾样本。使用储存的血清样本对回顾性调查疟疾流行率的研究也具有重要意义。需要使用血清和全血标本进行实地研究，以验证该技术，以便使这些方法适应临床应用。
Pooling clinical specimens reduces the number of assays needed when screening for infectious diseases. Polymerase chain reaction (PCR)-based assays are the most sensitive tests to diagnose malaria, but its high cost limits its use. We adapted a pooling platform that could reduce the number of assays needed to detect malaria infection. To evaluate this platform, two sets of 1.00 serum samples, with 1% and 5% malaria prevalence, were tested. DNA, extracted from pooled samples, was amplified by malaria-specific PCR. Additional validation was performed by determining the level of PCR detection based on 1:10 and 1:100 dilution. The platform correctly detected all malaria samples in the two test matrices. The use of stored serum samples also has important implications for studies investigating malaria prevalence rates retrospectively. Field studies, using serum and whole blood specimens, are needed to validate this technique for the adaptation of these methods for clinical utility.