Comparison of phytohormone biosynthesis and signal transduction pathways in developing and abortive hazelnut ovules
Comparison of phytohormone biosynthesis and signal transduction pathways in developing and abortive hazelnut ovules
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发育和败育榛子胚珠植物激素生物合成和信号转导途径的比较
DOI：
10.1007/s10725-016-0196-5
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发表时间：
2017-01-01
影响因子：
4.2
通讯作者：
Ming, Yuetong
中科院分区：
文献类型：
--
作者：
Cheng, Yunqing;Zhao, Yixin;Ming, Yuetong
在榛子中，胚珠败育与空坚果的形成有关，导致严重的产量损失。植物激素在植物生长发育中发挥着重要作用；然而，它们调节榛子胚珠败育的机制在很大程度上尚不清楚。 RNA-seq 通过京都基因与基因组百科全书 (KEGG) 通路注释鉴定了榛子发育中和败育胚珠之间的 1209 个差异表达基因。经过途径富集分析后，发现激素信号转导（ko04075）和几种植物激素生物合成途径显着富集。与发育中的胚珠相比，败育胚珠中吲哚-3-乙酸（IAA）、赤霉素（GA）、细胞分裂素（CTK）和水杨酸（SA）的水平显着降低，而脱落酸（ABA）、乙烯（ETH）和茉莉酸（JA）的水平显着升高。与这些结果一致，参与植物激素生物合成的一些重要限速酶，包括 9-顺式环氧类胡萝卜素双加氧酶、ACC 合酶和磷脂酶 A1 在败育胚珠中显着上调。 ETH、JA和ABA信号转导通路中的一些关键基因高度上调，包括ETH信号通路中的乙烯反应性转录因子1（ERF1/2）和丝氨酸/苏氨酸蛋白激酶CTR1，JA信号通路中的茉莉酸ZIM结构域含蛋白（JAZ）和转录因子MYC2，以及ABA信号通路中的ABA反应性元件结合因子（ABF）。 JA 信号通路。因此，ABA、ETH 和 JA 信号在败育胚珠中比在发育胚珠中更活跃。 q-RT-PCR显示NCED、ABF、ACS、ERF1/2、PLA1和MYC2的表达水平与差异表达基因(DEG)分析结果一致。我们的数据表明多种植物激素信号通路参与榛子胚珠的发育。 IAA、GA、CTK 和 SA 生物合成和信号通路的抑制以及 ABA、ETH 和 JA 生物合成和信号通路的激活可能导致榛子败育胚珠的形成。
In hazelnuts, ovule abortion is associated with the formation of empty nuts, resulting in serious yield losses. Phytohormones play important roles in plant growth and development; however, the mechanisms through which they regulate ovule abortion in hazelnut are largely unknown. RNA-seq identified 1209 differentially expressed genes with Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway annotation between developing and abortive ovules in hazelnut. Hormone signal transduction (ko04075), and several phytohormone biosynthesis pathways, were found to be significantly enriched after pathway enrichment analysis. Compared with developing ovules, the levels of indole-3-acetic acid (IAA), gibberellins (GA), cytokinin (CTK), and salicylic acid (SA) were found to be significantly lower in abortive ovules, while levels of abscisic acid (ABA), ethylene (ETH), and jasmonic acid (JA) were found to be significantly higher. Consistent with these results, some important rate-limiting enzymes involved in the biosynthesis of phytohormones, including 9-cis-epoxycarotenoid dioxygenase, ACC synthase, and phospholipase A1 were significantly up-regulated in abortive ovules. Some key genes in the ETH, JA, and ABA signal transduction pathways were highly upregulated, including ethylene-responsive transcription factor 1 (ERF1/2) and the serine/threonine-protein kinase CTR1 in the ETH signal pathway, jasmonate ZIM domain-containing protein (JAZ) and the transcription factor MYC2 in the JA signal pathway, and ABA responsive element binding factor (ABF) in the JA signal pathway. Thus, ABA, ETH, and JA signaling were more active in abortive ovules than in developing ovules. Expression levels ofNCED, ABF, ACS, ERF1/2, PLA1, andMYC2revealed by q-RT-PCR were consistent with the results obtained by differentially expressed genes (DEG) analysis. Our data suggest that multiple phytohormone signaling pathways are involved in the development of hazelnut ovules. Inhibition of IAA, GA, CTK, and SA biosynthesis and signaling pathways, and activation of ABA, ETH, and JA biosynthesis and signaling pathways may contribute to the formation of abortive ovules in hazelnut.