CHARACTERIZATION AND SPATIAL-DISTRIBUTION OF THE ELAV PROTEIN DURING DROSOPHILA-MELANOGASTER DEVELOPMENT
CHARACTERIZATION AND SPATIAL-DISTRIBUTION OF THE ELAV PROTEIN DURING DROSOPHILA-MELANOGASTER DEVELOPMENT
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DOI：
10.1002/neu.480220503
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发表时间：
1991-07-01
期刊：
影响因子：
--
通讯作者：
WHITE, K
中科院分区：
文献类型：
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作者：
ROBINOW, S;WHITE, K
果蝇胚胎致死异常视觉系统（elav）基因是神经系统发育和维持所必需的。该基因座的转录本在所有发育阶段的神经系统中无处不在。该基因的产物ELAV蛋白与已知的RNA结合蛋白具有同源性。在所有发育阶段使用针对大肠杆菌中制备的杂合蛋白产生的抗体研究ELAV蛋白的定位。一般而言，这些数据与先前的结果一致，并证明（1）在发育中的胚胎神经系统中检测到ELAV蛋白的时间与第一个神经元的出生一致，（2）ELAV蛋白首先在胚胎、幼虫、蛹和成虫的中枢和外周神经系统的大多数神经元中检测到，（3）ELAV蛋白似乎定位于细胞核，（4）在成神经细胞或可识别的神经胶质中未检测到ELAV蛋白。这些数据还提供了关于elav表达的新信息，并表明（1）ELAV不在神经节母细胞（GMC）中表达，（2）虽然ELAV蛋白定位于细胞核，但它并不均匀地分布在整个结构中，（3）其他果蝇物种确实表达ELAV样抗原。我们建议，elav基因提供了一个神经元管家功能，需要成功的转录后处理的转录本从一组基因的功能是需要适当的神经元的发育和维护。
The embryonic lethal abnormal visual system (elav) gene of Drosophila melanogaster is required for the development and maintenance of the nervous system. Transcripts from this locus are distributed ubiquitously throughout the nervous system at all developmental stages. A product of this gene, the ELAV protein, has homology to known RNA binding proteins. The localization of the ELAV protein was studied in all developmental stages using antibodies that were generated against a hybrid protein made in Escherichia coli. In general, these data are consistent with previous results and demonstrate that (1) the ELAV protein is detected in the developing embryonic nervous system at a time coincident with the birth of the first neurons, (2) the ELAV protein is first detected in the majority of neurons of the central and peripheral nervous systems of embryos, larvae, pupae, and adults, (3) the ELAV protein appears to be localized to the nucleus, and (4) the ELAV protein is not detected in neuroblasts or identifiable glia. These data also provide new information concerning elav expression and show that (1) ELAV is not expressed in the ganglion mother cells (GMCs), (2) while the ELAV protein is localized to the nucleus, it is not uniformly distributed throughout this structure, and (3) other Drosophila species do express an ELAV-like antigen. We propose that the elav gene provides a neuronal-housekeeping function that is required for the successful posttranscriptional processing of transcripts from a set of genes the function of which is required for proper neuronal development and maintenance.