Live imaging of adult neural stem cell behavior in the intact and injured zebrafish brain
Live imaging of adult neural stem cell behavior in the intact and injured zebrafish brain
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DOI：
10.1126/science.aaa2729
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发表时间：
2015-05-15
期刊：
影响因子：
56.9
通讯作者：
Ninkovic, Jovica
中科院分区：
文献类型：
--
作者：
Barbosa, Joana S.;Sanchez-Gonzalez, Rosario;Ninkovic, Jovica
成体神经干细胞是修复受损脑组织的来源。我们使用重复成像来跟踪体内完整和受伤的成年斑马鱼端脑中的单个干细胞，发现神经元是通过干细胞直接转化为有丝分裂后神经元以及通过中间祖细胞放大神经元输出而产生的。我们观察到消耗干细胞的直接转化以及不对称和对称自我更新分裂的不平衡，导致干细胞随着时间的推移而耗尽。脑损伤后，神经元祖细胞被募集到损伤部位。这些祖细胞是通过消耗干细胞池的对称分裂产生的，这是完整端脑中不存在的神经发生模式。我们的分析揭示了干细胞在再生过程中产生额外神经元的行为变化。
Adult neural stem cells are the source for restoring injured brain tissue. We used repetitive imaging to follow single stem cells in the intact and injured adult zebrafish telencephalon in vivo and found that neurons are generated by both direct conversions of stem cells into postmitotic neurons and via intermediate progenitors amplifying the neuronal output. We observed an imbalance of direct conversion consuming the stem cells and asymmetric and symmetric self-renewing divisions, leading to depletion of stem cells over time. After brain injury, neuronal progenitors are recruited to the injury site. These progenitors are generated by symmetric divisions that deplete the pool of stem cells, a mode of neurogenesis absent in the intact telencephalon. Our analysis revealed changes in the behavior of stem cells underlying generation of additional neurons during regeneration.