The Arabidopsis ATR1 Myb transcription factor controls indolic glucosinolate homeostasis
The Arabidopsis ATR1 Myb transcription factor controls indolic glucosinolate homeostasis
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DOI：
10.1104/pp.104.054395
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发表时间：
2005-01-01
期刊：
影响因子：
7.4
通讯作者：
Bender, J
中科院分区：
文献类型：
--
作者：
Celenza, JL;Quiel, JA;Bender, J
植物从氨基酸色氨酸 (Trp) 中衍生出许多重要的次生代谢物，包括生长调节剂吲哚-3-乙酸 (IAA) 以及针对病原体和食草动物的防御化合物。在之前的工作中，我们发现拟南芥Myb转录因子ATR1的显性过表达等位基因atr1D激活色氨酸合成基因以及色氨酸代谢基因CYP79B2、CYP79B3和CYP83B1的表达，这些基因编码参与IAA和吲哚芥子油苷生产的酶 (IG) 抗草食动物化合物。在这里，我们发现 ATR1 过度表达会导致 IAA 和 IG 水平升高。此外，我们发现 atr1 功能丧失突变会损害 IG 合成基因的表达并导致 IG 水平降低。此外，atr1 缺陷突变会抑制 cyp83B1 突变背景中的 Trp 基因失调。总之，这项工作表明 ATR1 是色氨酸代谢的关键稳态调节因子，并表明可以操纵 ATR1 来协调控制合成 IG 的一系列酶。
Plants derive a number of important secondary metabolites from the amino acid tryptophan (Trp), including the growth regulator indole-3-acetic acid (IAA) and defense compounds against pathogens and herbivores. In previous work, we found that a dominant overexpression allele of the Arabidopsis (Arabidopsis thaliana) Myb transcription factor ATR1, atr1D, activates expression of a Trp synthesis gene as well as the Trp-metabolizing genes CYP79B2, CYP79B3, and CYP83B1, which encode enzymes implicated in production of IAA and indolic glucosinolate (IG) antiherbivore compounds. Here, we show that ATR1 overexpression confers elevated levels of IAA and lGs. In addition, we show that an atr1 loss-of-function mutation impairs expression of IG synthesis genes and confers reduced IG levels. Furthermore, the atr1-defective mutation suppresses Trp gene dysregulation in a cyp83B1 mutant background. Together, this work implicates ATR1 as a key homeostatic regulator of Trp metabolism and suggests that ATR1 can be manipulated to coordinately control the suite of enzymes that synthesize IGs.