Occurrence of Cu,Zn-Superoxide Dismutase in the Intrathylakoid Space of Spinach Chloroplasts
Occurrence of Cu,Zn-Superoxide Dismutase in the Intrathylakoid Space of Spinach Chloroplasts
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菠菜叶绿体类囊体间隙中铜、锌超氧化物歧化酶的存在
DOI：
10.1093/oxfordjournals.pcp.a076802
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发表时间：
1984
影响因子：
4.9
通讯作者：
K. Asada
中科院分区：
文献类型：
--
作者：
T. Hayakawa;S. Kanematsu;K. Asada
从完整的菠菜叶绿体中获得的类囊体没有表现出超氧化物歧化酶（SOD）的活性，但在Triton X-100的存在下检测到Cu，Zn-和Mn-SOD的活性。用Yeda压力池处理类囊体后，离心分离类囊体膜和管腔部分。Cu，Zn-SOD主要存在于管腔部分。Mn-SOD在类囊体组分中仅在加入1%Triton X-100后才被检测到。抗菠菜Cu，Zn-SOD抗体不与类囊体中的Cu，Zn-SOD发生相互作用，除非加入Triton。这些结果表明，Cu，Zn-SOD除存在于叶绿体基质外，还存在于叶绿体内腔，Mn-SOD则与类囊体膜结合。
Thylakoids obtained from intact spinach chloroplasts showed no superoxide dismutase (SOD) activity, but Cu,Zn- and Mn-SOD activities were detected in the presence of Triton X-100. Thylakoid membranes and the lumen fraction were separated by centrifugation after treatment of the thylakoids with a Yeda pressure cell. Cu,Zn-SOD was found in the lumen fraction. Mn-SOD was detected in the thylakoid fraction only after addition of 1% Triton X-100. Antibody against spinach Cu,Zn-SOD did not interact with the latent Cu,Zn-SOD in the thylakoids unless Triton was added. These results indicate that Cu,Zn-SOD occurs in the lumen in addition to the stroma of spinach chloroplasts, and Mn-SOD binds to the thylakoid membranes.