Post-translational modifications in the active site region of methyl-coenzyme M reductase from methanogenic and methanotrophic archaea
Post-translational modifications in the active site region of methyl-coenzyme M reductase from methanogenic and methanotrophic archaea
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DOI：
10.1111/j.1742-4658.2007.06016.x
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发表时间：
2007-09-01
期刊：
影响因子：
5.4
通讯作者：
Thauer, Rudolf K.
中科院分区：
文献类型：
--
作者：
Kahnt, Joerg;Buchenau, Baerbel;Thauer, Rudolf K.
甲基辅酶M还原酶（MCR）催化产甲烷古菌中的甲烷形成步骤。甲烷热杆菌同工酶Ⅰ的活性部位含有一个硫代肽键和四个甲基化氨基酸。我们在这里报告说，MCR从所有甲烷菌调查含有硫代肽键，但不同的酶在其翻译后甲基化。MS分析包括来自马勃氏甲烷热杆菌的MCR I和MCR II，来自詹氏甲烷球菌和嗜热甲烷球菌的MCR I，以及来自甲烷球菌、坎氏甲烷吡喃菌和巴克氏甲烷八叠球菌的MCR。还分析了从黑海垫中分离的两个MCR，其中主要含有ANME-1簇的嗜甲烷古菌。
Methyl-coenzyme M reductase (MCR) catalyzes the methane-forming step in methanogenic archaea. Isoenzyme I from Methanothermobacter marburgensiswas shown to contain a thioxo peptide bond and four methylated amino acids in the active site region. We report here that MCRs from all methanogens investigated contain the thioxo peptide bond, but that the enzymes differ in their post-translational methylations. The MS analysis included MCR I and MCR II from Methanothermobacter marburgensis, MCR I from Methanocaldococcus jannaschii and Methanoculleus thermophilus, and MCR from Methanococcus voltae, Methanopyrus kandleri and Methanosarcina barkeri. Two MCRs isolated from Black Sea mats containing mainly methanotrophic archaea of the ANME-1 cluster were also analyzed.