DIRECT EVIDENCE FOR INVIVO HYDROXYL-RADICAL GENERATION IN EXPERIMENTAL IRON OVERLOAD - AN ESR SPIN-TRAPPING INVESTIGATION
DIRECT EVIDENCE FOR INVIVO HYDROXYL-RADICAL GENERATION IN EXPERIMENTAL IRON OVERLOAD - AN ESR SPIN-TRAPPING INVESTIGATION
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DOI：
10.1073/pnas.88.19.8440
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发表时间：
1991-10-01
影响因子：
11.1
通讯作者：
MASON, RP
中科院分区：
文献类型：
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作者：
BURKITT, MJ;MASON, RP
虽然羟基自由基通常是牵连的物种负责在铁超载条件下的氧化损伤的启动，没有ESR证据的自由基在体内形成的报告。 我们采用了二级自由基捕获技术，其中羟基自由基与二甲基亚砜反应形成甲基自由基，然后检测到其加合物的自旋陷阱N-叔丁基-α-苯基硝酮在动物的胆汁中给予硫酸亚铁的胃内剂量。 通过同位素取代技术验证了该加合物的身份。 我们表明，除非采取措施，以消除铁排泄在胆汁中的治疗动物，铁，氧，二甲亚砜，N-叔丁基-α-苯基硝酮，和胆汁成分之间的反应导致在样品采集过程中形成的文物。
Although the hydroxyl radical is often implicated as the species responsible for the initiation of oxidative damage in iron-overload conditions, no ESR evidence for the formation of the radical in vivo has been reported. We have employed a secondary radical-trapping technique in which the hydroxyl radical reacts with dimethyl sulfoxide to form the methyl radical, which is then detected as its adduct of the spin trap N-t-butyl-alpha-phenylnitrone in the bile of animals given an intragastric dose of ferrous sulfate. The identity of this adduct was verified by isotope-substitution techniques. We show that unless measures are taken to inactivate the iron excreted in the bile of treated animals, reactions between iron, oxygen, dimethyl sulfoxide, N-t-butyl-alpha-phenylnitrone, and bile components lead to the formation of artifacts during sample collection.