Differential expression of NMDA receptor subunits and splice variants among the CA1, CA3 and dentate gyrus of the adult rat
Differential expression of NMDA receptor subunits and splice variants among the CA1, CA3 and dentate gyrus of the adult rat
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DOI：
10.1016/j.molbrainres.2004.12.005
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发表时间：
2005-04-27
期刊：
影响因子：
--
通讯作者：
Browning, MD
中科院分区：
文献类型：
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作者：
Coultrap, SJ;Nixon, KM;Browning, MD
海马n -甲基- d -天冬氨酸（NMDA）型谷氨酸受体是记忆形成和兴奋毒性的重要介质。据认为，海马CA1、CA3和齿状回区域的谷氨酸能神经元对记忆形成的贡献不同，对兴奋毒性的敏感性也不同。NMDA受体的亚基和/或剪接变体组成控制着受体功能的许多方面，如配体亲和力、钙通透性和通道动力学，以及与细胞内锚定蛋白和调节蛋白的相互作用。因此，对海马亚区中NMDA受体依赖性过程（如突触可塑性和兴奋毒性）差异的一种可能解释是，它们在亚基和/或剪接变体表达上存在差异。在这里，我们报道了NMDA受体亚基NR1和NR2B，以及NR1亚基的四个剪接变体盒在海马CA1、CA3和齿状回中差异表达。AMPA受体亚基GluR1和GluR2的表达也不同。这些差异可能导致功能差异，如海马亚区之间存在的兴奋毒性和突触可塑性。(c) 2005 Elsevier B.V.版权所有
N-Methyl-D-aspartate (NMDA)-type glutamate receptors in the hippocampus are important mediators of both memory formation and excitotoxicity. It is thought that glutamatergic neurons of the CA1, CA3 and dentate gyrus regions of the hippocampus contribute differentially to memory formation and are differentially sensitive to excitotoxicity. The subunit and/or splice variant composition of the NMDA receptor controls many aspects of receptor function such as ligand affinity, calcium permeability and channel kinetics, as well as interactions with intracellular anchoring and regulatory proteins. Thus, one possible explanation of the differences in NMDA receptor-dependent processes, such as synaptic plasticity and excitotoxicity, among the hippocampal sub-regions is that they differ in subunit and/or splice variant expression. Here we report that the NMDA receptor subunits NR1 and NR2B, along with the four splice variant cassettes of the NR1 subunit are differentially expressed in the CA1, CA3 and dentate gyros of the hippocampus. Expression of the AMPA receptor subunits GluR1 and GluR2 also differ. These differences may contribute to functional differences, such as with excitotoxicity and synaptic plasticity, that exist between the sub-regions of the hippocampus. (c) 2005 Elsevier B.V. All rights reserved.