Comparison of in vitro antifungal activities of amphotericin B lipid complex with itraconazole against 708 clinical yeast isolates and opportunistic moulds determined by national committee for clinical laboratory standards methods M27-A and M38-P
Comparison of in vitro antifungal activities of amphotericin B lipid complex with itraconazole against 708 clinical yeast isolates and opportunistic moulds determined by national committee for clinical laboratory standards methods M27-A and M38-P
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DOI：
10.1159/000066764
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发表时间：
2002-12-01
期刊：
影响因子：
3.3
通讯作者：
Quindós, G
中科院分区：
文献类型：
--
作者：
Carrillo-Muñoz, AJ;Ruesga, M;Quindós, G
我们采用微量稀释法（国家临床实验室标准委员会 M27-A 和 M38-P）比较了两性霉素 B 脂质复合物 (ABLC) 与伊曲康唑 (ITZ) 对 535 种酵母菌株和 173 种机会性丝状真菌的体外抗真菌活性。 ITZ 和 ABLC 的总体几何平均 MIC 分别为 0.13 mug/ml 和 0.177 mug/ml，两种药物针对酵母分离株的 MIC50 均为 0.125 mug/ml。当针对白色念珠菌、近平滑念珠菌、新型隐球菌、克柔念珠菌、光滑念珠菌和热带念珠菌进行测试时，ITZ 与 ABLC 相比具有相似或稍优的功效。 ITZ 对抗光滑念珠菌的有效性较低（MIC90 2 微克/毫升，ABLC 为 0.5 微克/毫升）。对于烟曲霉，ITZ 的活性优于 ABLC（MIC90 分别为 1 和 16 mug/ml）； ABLC 和 ITZ 的黑曲霉 MIC90 分别为 4 和 2 微克/毫升。 Scedosporium spp.对 ABLC 和 ITZ 均表现出低敏感性。总之，ABLC 和 ITZ 是治疗严重真菌感染的有用替代方案。抗真菌药物的选择应考虑毒理学和药理学特性以及成本/效益关系，并以分离株的敏感性为依据。版权所有 (C) 2002 S. Karger AG，巴塞尔。
We compared the in vitro antifungal activity of amphotericin B lipid complex (ABLC) with that of itraconazole (ITZ) against 535 yeast strains and 173 opportunistic filamentous fungi by using a microdilution method (National Committee for Clinical Laboratory Standards M27-A and M38-P). The overall geometric mean MIC was 0.13 mug/ml and 0.177 mug/ml for ITZ and ABLC, respectively, and the MIC50 was 0.125 mug/ml for both agents against yeast isolates. ITZ had a similar or slightly superior efficacy compared to ABLC when tested against Candida albicans, Candida parapsilosis, Cryptococcus neoformans, Candida krusei, Candida glabrata and Candida tropicalis. Effectiveness against C. glabrata was lower for ITZ (MIC90 2 mug/ml, and for ABLC, 0.5 mug/ml). For Aspergillus fumigatus, activity of ITZ was superior in comparison with ABLC (MIC90 1 and 16 mug/ml, respectively); MIC90 for Aspergillus niger was 4 and 2 mug/ml for ABLC and ITZ, respectively. Scedosporium spp. showed a low susceptibility to both ABLC and ITZ. In conclusion, ABLC and ITZ are useful alternatives for the treatment of severe fungal infections. The selection of an antifungal agent should be made considering the toxicological and pharmacological properties and cost/benefit relationship and be supported by the susceptibility of the isolate. Copyright (C) 2002 S. Karger AG, Basel.