Comparative transcriptome analysis of TLR8 signaling cells revealed the porcine TLR8 specific differentially expressed genes
Comparative transcriptome analysis of TLR8 signaling cells revealed the porcine TLR8 specific differentially expressed genes
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TLR8信号细胞的比较转录组分析揭示了猪TLR8特异性差异表达基因
DOI：
10.1016/j.dci.2019.05.004
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发表时间：
2019-09-01
影响因子：
2.9
通讯作者：
Zhu, Jianzhong
中科院分区：
文献类型：
--
作者：
Ao, Da;Xia, Pengpeng;Zhu, Jianzhong
TLRs是第一个发现的模式识别受体(PRRs)家族。它们识别病原体相关分子模式(PAMP)并启动保护性免疫反应。TLR8作为主要的内溶酶体TLR，近年来在结构和功能方面重新引起人们的关注。我们先前发现TLR8被TLR7特异性激动剂咪喹莫特(R837)激活。因此，我们接下来通过对猪TLR8和人TLR8(HTLR8)的平行转录组学分析来鉴定猪TLR8(PTLR8)特异的下游差异表达基因(DEG)，该转录组信号传递由TLR8激动剂Resiquimod(R848)激活的稳定的NF-kappa B报告细胞。结果表明，这两个TLR8核因子-kappaB报告细胞可以概括pTLR8和hTLR8的物种特异性活性，转录组分析发现R848激活了一些pTLR8特异的deg，其中一些基因在猪肺泡巨噬细胞(PAM)中得到了证实，但在人类细胞中没有表达。
TLRs are the first discovered family of pattern recognition receptors (PRRs). They recognize pathogen associated molecular patterns (PAMPs) and initiate protective immune response. TLR8 as the main endolysosomal TLR, has recently regained attention especially for its structure and function. We previously found TLR8 exhibits species specific activation by TLR7 specific agonist, Imiquimod (R837). Thus, we next initiated the identification of porcine TLR8 (pTLR8) specific downstream differentially expressed genes (DEGs) by parallel transcriptome analysis of porcine TLR8 and human TLR8 (hTLR8) signaling stable NF-kappa B reporter cells activated by TLR8 agonist Resiquimod (R848). It turned out that the two TLR8 NF-kappa B reporter cells can recapitulate the species specific activity of pTLR8 and hTLR8, transcriptome analysis revealed a number of pTLR8 specific DEGs activated by R848, and some of these gene expressions were confirmed in porcine alveolar macrophages (PAMs) but not occurred in human cell types.