Microbiological aspects of the investigation that traced the 1998 outbreak of listeriosis in the United States to contaminated hot dogs and establishment of molecular subtyping-based surveillance for Listeria monocytogenes in the PulseNet network
Microbiological aspects of the investigation that traced the 1998 outbreak of listeriosis in the United States to contaminated hot dogs and establishment of molecular subtyping-based surveillance for Listeria monocytogenes in the PulseNet network
复制标题
DOI：
10.1128/jcm.43.5.2350-2355.2005
复制
发表时间：
2005-05-01
影响因子：
9.4
通讯作者：
Swaminathan, B
中科院分区：
文献类型：
--
作者：
Graves, LM;Hunter, SB;Swaminathan, B
1998年，美国多州暴发了一次猪链球菌病，发病日期为8月至12月。疫情导致居住在24个州的108人患病，造成14人死亡，4人流产或死产。田纳西州和纽约的公共卫生官员发现了这次疫情，他们观察到，在他们的州，霍乱病例比预期的要多得多。随后，疾病控制和预防中心（CDC）开始通过血清分型和使用脉冲场凝胶电泳（PFGE）的限制性片段长度多态性分析对单核细胞增生李斯特菌的临床分离株进行实验室表征。在本研究中，暴发相关分离株定义为当其DNA被AscI和ApaI限制性内切酶限制时，具有特异性AscI-PFGE图谱和不可区分或高度相似（26条带中不超过2条带差异）的ApaI-PFGE图谱的分离株。及时获得分子分型结果使流行病学家能够将暴发病例与同一地理区域内时间相关的散发病例分开，并有助于将单一商业设施生产的受污染热狗确定为暴发源。在这次暴发的调查过程中，建立了一个标准化的L亚型分型方案。通过PFGE对单核细胞增生进行分型，并分发给参与CDC PulseNet网络的公共卫生实验室;要求这些实验室开始常规PFGE分型L。单核细胞增多症
A multistate outbreak of listeriosis occurred in the United States in 1998 with illness onset dates between August and December. The outbreak caused illness in 108 persons residing in 24 states and caused 14 deaths and four miscarriages or stillbirths. This outbreak was detected by public health officials in Tennessee and New York who observed significant increases over expected listeriosis cases in their states. Subsequently, the Centers for Disease Control and Prevention (CDC) began laboratory characterization of clinical isolates of Listeria monocytogenes by serotyping and restriction fragment length polymorphism analysis using pulsed-field gel electrophoresis (PFGE). For the purpose of this investigation, outbreak-related isolates were defined as those that had a specific AscI-PFGE pattern and indistinguishable or highly similar (no more than 2 band difference in 26 bands) ApaI-PFGE patterns when their DNA was restricted by AscI and ApaI restriction enzymes. Timely availability of molecular subtyping results enabled epidemiologists to separate outbreak cases from temporally associated sporadic cases in the same geographic areas and facilitated the identification of contaminated hot dogs manufactured at a single commercial facility as the source of the outbreak. During the investigation of this outbreak, a standardized protocol for subtyping L. monocytogenes by PFGE was developed and disseminated to public health laboratories participating with CDC's PulseNet network; these laboratories were requested to begin routine PFGE subtyping of L. monocytogenes.