Monitoring local synaptic activity with astrocytic patch pipettes
Monitoring local synaptic activity with astrocytic patch pipettes
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DOI：
10.1038/nprot.2012.140
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发表时间：
2012-12-01
期刊：
影响因子：
14.8
通讯作者：
Rusakov, Dmitri A.
中科院分区：
文献类型：
--
作者：
Henneberger, Christian;Rusakov, Dmitri A.
星形胶质细胞和神经元之间的快速信号交换已成为大脑神经通讯的关键参与者。为了了解所涉及的机制，在监测附近突触的活动的同时接触单个星形胶质细胞通常很重要。使用标准电生理学工具实现这一目标并不总是可行的。这里介绍的协议可以使用本地星形胶质细胞的全细胞电流钳记录来监测突触活动。这种方法利用了电被动星形胶质细胞的低输入电阻允许细胞外电流以相对较小的衰减穿过星形胶质细胞膜的事实。一旦切片准备就绪，根据实验设计，需要大约 30 分钟到几个小时来实施该协议，这与其他膜片钳技术类似。这里介绍的技术可用于直接访问单个星形胶质细胞的细胞内介质，同时检查其附近功能的突触。
Rapid signal exchange between astroglia and neurons has emerged as a key player in neural communication in the brain. To understand the mechanisms involved, it is often important to have access to individual astrocytes while monitoring the activity of nearby synapses. Achieving this with standard electrophysiological tools is not always feasible. The protocol presented here enables the monitoring of synaptic activity using whole-cell current-clamp recordings from a local astrocyte. This approach takes advantage of the fact that the low input resistance of electrically passive astroglia allows extracellular currents to pass through the astrocytic membrane with relatively little attenuation. Once the slice preparation is ready, it takes similar to 30 min to several hours to implement this protocol, depending on the experimental design, which is similar to other patch-clamp techniques. The technique presented here can be used to directly access the intracellular medium of individual astrocytes while examining synapses functioning in their immediate proximity.