Phenotypic heterogeneity within microbial populations at the single-cell level investigated by confocal Raman microspectroscopy
Phenotypic heterogeneity within microbial populations at the single-cell level investigated by confocal Raman microspectroscopy
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DOI：
10.1039/b822574e
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发表时间：
2009-01-01
期刊：
影响因子：
4.2
通讯作者：
Naumann, Dieter
中科院分区：
文献类型：
--
作者：
Hermelink, Antje;Brauer, Angelika;Naumann, Dieter
遗传同质微生物培养物中的单个细胞表现出明显的表型异质性，这被认为是支持整个种群的适应性。单细胞水平上的异质性通常在微生物种群的常规研究中被掩盖，这些研究依赖于样品中数千或数百万个细胞的平均数据。在这里，我们介绍共焦拉曼显微光谱作为一种方法，用于调查和说明的空间异质性的微生物细胞群体。通过使用三种不同的测试生物作为模型系统，我们显示出明显的细胞异质性，即使在实验室条件下培养的菌落。
Single cells in genetically homogeneous microbial cultures exhibit marked phenotypic heterogeneity that is considered to bolster the fitness of the whole population. Heterogeneity on the single-cell level is typically masked in conventional studies of microbial populations, which rely on data averaged across thousands or millions of cells in a sample. Here we introduce confocal Raman microspectroscopy as a method for investigating and illustrating the spatial heterogeneity of microbial cell populations. By the use of three different test organisms as model systems, we show pronounced cellular heterogeneity even in colonies cultivated under laboratory conditions.