Combined treatment with capsaicin and resveratrol enhances neuroprotection against glutamate-induced toxicity in mouse cerebral cortical neurons
Combined treatment with capsaicin and resveratrol enhances neuroprotection against glutamate-induced toxicity in mouse cerebral cortical neurons
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DOI：
10.1016/j.fct.2012.08.040
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发表时间：
2012-11-01
影响因子：
4.3
通讯作者：
Nam, Sang-Yoon
中科院分区：
文献类型：
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作者：
Lee, Jong-Geol;Yon, Jung-Min;Nam, Sang-Yoon
辣椒素和白藜芦醇作为天然产物具有多种有益作用。然而，辣椒素也是一种神经毒剂，使其对神经系统的影响令人困惑。本研究的目的是探讨辣椒素和/或白藜芦醇是否对谷氨酸(Glu)诱导的神经毒性有保护作用。用辣椒素和/或白藜芦醇处理胎龄15~16天的ICR胎鼠大脑皮层神经元24小时后，细胞存活率显著降低，但辣椒素和白藜芦醇处理后细胞存活率显著增加，两者联合作用的细胞存活率最高。这两种植物化学物质的联合治疗也显著减少了谷氨酸诱导的活性氧的产生和神经元的凋亡。辣椒素和/或白藜芦醇可显著恢复谷胱甘肽过氧化物酶、铜/锌、锰超氧化物歧化酶和Bc l-x(L)的低表达及白介素1β和肿瘤坏死因子-α的高表达。这些结果表明，辣椒素和白藜芦醇对谷氨酸诱导的毒性具有神经保护作用，这两种植物化学物质的联合治疗可以增强神经保护作用，这表明在治疗神经退行性疾病方面有有用的应用。(C)2012爱思唯尔有限公司。保留所有权利。
Capsaicin and resveratrol as natural products have a variety of beneficial effects. However, capsaicin is also a neurotoxic agent, rendering its effect on the nervous system confusing. The aim of this study was to investigate whether capsaicin and/or resveratrol have a protective effect against glutamate (Glu)-induced neurotoxicity. After exposure to glutamate for 15 min, cerebral cortical neurons of ICR mouse fetuses on embryonic days 15-16 were post-treated with capsaicin and/or resveratrol for 24 h. Glu induced a significant reduction in cell viability, but the cell viability increased significantly with capsaicin or resveratrol treatment and further was highest in the neurons co-treated with both phytochemicals. Glu-induced reactive oxygen species generation and apoptotic neuronal death also significantly decreased by a combined treatment with both phytochemicals. Due to Glu insults, the reduced mRNA levels of cytoplasmic glutathione peroxidase, copper/zinc and manganese superoxide dismutases, and Bcl-x(L) and the overexpressed mRNA levels of interleukin-1 beta and tumor necrosis factor-alpha were significantly restored by post-treatment of capsaicin and/or resveratrol. These findings indicate that capsaicin and resveratrol are neuroprotective against Glu-induced toxicity and that the combined treatment of both phytochemicals can enhance the neuroprotection, suggesting a useful therapeutic application in the treatment of neurodegenerative disorders. (C) 2012 Elsevier Ltd. All rights reserved.